Rat CD45R MicroBeads have been developed for positive selection or depletion of rat B cells from bone marrow, peripheral lymphoid organs, and non-lymphoid tissue.

Data and images for CD45R MicroBeads, rat

Figures

Figure 1

View details
Isolation of B cells from rat spleen using CD45R MicroBeads, a MiniMACS™ Separator, and an MS Column.

Figure 1

Isolation of B cells from rat spleen using CD45R MicroBeads, a MiniMACS™ Separator, and an MS Column.

Specifications for CD45R MicroBeads, rat

Overview

Rat CD45R MicroBeads have been developed for positive selection or depletion of rat B cells from bone marrow, peripheral lymphoid organs, and non-lymphoid tissue.

Detailed product information

Background information

The CD45R antigen is expressed throughout B cell development from the pro-B cell stage on, but it is down-regulated during the differentiation of B cells into plasma cells. Expression levels of CD45R are different among distinct B cell subpopulations. Memory B cells are CD45R
low/-
. CD45R is also weakly expressed on a small subset of peripheral T cells, but is not detected on thymocytes.

Downstream applications

B cells isolated from bone marrow or peripheral lymphoid organs can be used for
in vitro
or
ex vivo
analysis of B cell lymphopoiesis and B cell migration. B cells purified with CD45R MicroBeads can be used for studies on the mechanism and regulation of immunoglobulin gene rearrangement.

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.