Clone:
REAL131
Type of antibody:
Releasable antibodies, Primary antibodies, Recombinant antibodies
Applications:
FC, MICS, IHC, IF
Alternative names:
Bp50, CD40L receptor, CDW40

Extended validation for CD40 Antibody, anti-human,
REAlease
®

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
View details
Overlay histogram showing flow cytometric analysis of CD40 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD40-APC, clone (REAL131). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of CD40 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD40-APC, clone (REAL131). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD40 Antibody, anti-human,
REAlease
®

Overview

Clone REAL131 is an antibody fragment derived from the full CD40 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.
Clone REAL131 recognizes the human CD40 antigen, a transmembrane receptor of the tumor necrosis factor gene superfamily. CD40 is a co-stimulatory protein constitutively expressed by antigen presenting cells, including dendritic cells, B cells, and macrophages and on endothelial, smooth muscle cells, and fibroblasts. The binding of CD40 to CD154 (CD40L) on T helper cells activates antigen presenting cells and enhances the expression of cytokines, chemokines, matrix metalloproteinases, growth factors, and adhesion molecules. CD40 has been shown to interact with members of the TRAF family.
The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

Bp50, CD40L receptor, CDW40

Detailed product information

Technical specifications

CloneREAL131
Clonalitymonoclonal
Isotype controlControl Antibody
Hostcell line
Type of antibodyReleasable antibodies, Primary antibodies, Recombinant antibodies
Specieshuman
AntigenCD40
Alternative names of antigenBp50, CD40L receptor, CDW40
Distribution of antigendendritic cells, B cells, macrophages, endothelial cells, fibroblasts, smooth muscle
RRIDAB_2751270, AB_2751263, AB_2784185

References for CD40 Antibody, anti-human,
REAlease
®

Publications

  1. Stamenkovic, I. et al. (1989) A B-lymphocyte activation molecule related to the nerve growth factor receptor and induced by cytokines in carcinomas. EMBO J. 8(5): 1403-1410
  2. Sato, T. et al. (1995) A novel member of the TRAF family of putative signal transducing proteins binds to the cytosolic domain of CD40. FEBS Lett. 358(2): 113-118
  3. Chatzigeorgiou, A. et al. (2009) CD40/CD40L signaling and its implication in health and disease. Biofactors 35(6): 474-483

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REAlease
®

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