The CD4
+
CD25
+
Regulatory T Cell Isolation Kit has been developed for the positive selection of CD4
+
CD25
+
regulatory T cells (Treg) from rhesus monkey PBMCs.

Data and images for
CD4
+
CD25
+
Regulatory T Cell Isolation Kit
, non-human primate

Figures

Figure 1

Isolation of CD4
+
CD25
+
regulatory T cells from rhesus monkey PBMCs using the CD4
+
CD25
+
Regulatory T Cell Isolation Kit, an LD Column and two MS Columns, a MidiMACS™ Separator and a MiniMACS™ Separator.
PBMCs before separation
Pre-enriched CD4
+
T cells after depletion of non-CD4
+
T cells
View details

Figure 1

Isolation of CD4
+
CD25
+
regulatory T cells from rhesus monkey PBMCs using the CD4
+
CD25
+
Regulatory T Cell Isolation Kit, an LD Column and two MS Columns, a MidiMACS™ Separator and a MiniMACS™ Separator.
View details

Figure 1

Isolation of CD4
+
CD25
+
regulatory T cells from rhesus monkey PBMCs using the CD4
+
CD25
+
Regulatory T Cell Isolation Kit, an LD Column and two MS Columns, a MidiMACS™ Separator and a MiniMACS™ Separator.
Isolated CD4
+
CD25
+
regulatory T cells
View details

Figure 1

Isolation of CD4
+
CD25
+
regulatory T cells from rhesus monkey PBMCs using the CD4
+
CD25
+
Regulatory T Cell Isolation Kit, an LD Column and two MS Columns, a MidiMACS™ Separator and a MiniMACS™ Separator.

Specifications for
CD4
+
CD25
+
Regulatory T Cell Isolation Kit
, non-human primate

Overview

The CD4
+
CD25
+
Regulatory T Cell Isolation Kit has been developed for the positive selection of CD4
+
CD25
+
regulatory T cells (Treg) from rhesus monkey PBMCs.

Detailed product information

Background information

The kit contains a cocktail of biotinylated antibodies and Anti-Biotin MicroBeads for depletion of non-CD4
+
T cells and CD25 MicroBeads for subsequent positive selection of the CD4
+
CD25
+
regulatory T cells (Treg).
The role in the modulation of immune responses has been widely investigated for rodent and human CD4
+
CD25
+
Treg cells. A population of CD4
+
CD25
+
Tregs with a phenotype and function similar to the murine and human cells has also been described in non-human primates.

Downstream applications

CD4
+
CD25
+
regulatory T cells isolated with the CD4
+
CD25
+
Regulatory T Cell Isolation Kit can be used for further phenotypical and functional characterization.

Columns

For the first magnetic separation (depletion): LD or autoMACS
®
Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.

Resources for
CD4
+
CD25
+
Regulatory T Cell Isolation Kit
, non-human primate

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CD4
+
CD25
+
Regulatory T Cell Isolation Kit
, non-human primate

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