The CD34 MultiSort Kit has been developed for the positive selection of CD34
+
subpopulations by sequential sorting. CD34
+
hematopoietic stem and progenitor cells can be isolated from
  • peripheral blood,
  • bone marrow aspirate,
  • leukapheresis product,
  • cord blood,
  • differentiated ES and iPS cell cultures1.

Data and images for CD34 MultiSort Kit, human

Figures

Figure 1

CD34
+
HLA-DR– cells isolated from human PBMCs using the CD34 MultiSort Kit. Positive selection of CD34
+
cells from PBMCs was followed by the depletion of CD34
+
HLA-DR
+
cells using HLA-DR-FITC and Anti-FITC MicroBeads resulting in a population of CD34
+
HLA-DR
cells (red).
A:
B:
PBMCs before separation
CD34
+
cells
View details

Figure 1

CD34
+
HLA-DR– cells isolated from human PBMCs using the CD34 MultiSort Kit. Positive selection of CD34
+
cells from PBMCs was followed by the depletion of CD34
+
HLA-DR
+
cells using HLA-DR-FITC and Anti-FITC MicroBeads resulting in a population of CD34
+
HLA-DR
cells (red).
View details

Figure 1

CD34
+
HLA-DR– cells isolated from human PBMCs using the CD34 MultiSort Kit. Positive selection of CD34
+
cells from PBMCs was followed by the depletion of CD34
+
HLA-DR
+
cells using HLA-DR-FITC and Anti-FITC MicroBeads resulting in a population of CD34
+
HLA-DR
cells (red).
C:
CD34
+
HLA-DR
-
cells
View details

Figure 1

CD34
+
HLA-DR– cells isolated from human PBMCs using the CD34 MultiSort Kit. Positive selection of CD34
+
cells from PBMCs was followed by the depletion of CD34
+
HLA-DR
+
cells using HLA-DR-FITC and Anti-FITC MicroBeads resulting in a population of CD34
+
HLA-DR
cells (red).

Figure 2

View details
Step-by-step CD34 MultiSort separation procedure.

Figure 2

Step-by-step CD34 MultiSort separation procedure.

Figure 3

View details
CD34
+
lin
-
cells isolated from peripheral blood using CD34 MultiSort Kit, May-Grünwald-Giemsa stained. (Courtesy of Dr. Ziegler, Tübingen, Germany.)

Figure 3

CD34
+
lin
-
cells isolated from peripheral blood using CD34 MultiSort Kit, May-Grünwald-Giemsa stained. (Courtesy of Dr. Ziegler, Tübingen, Germany.)

Specifications for CD34 MultiSort Kit, human

Overview

The CD34 MultiSort Kit has been developed for the positive selection of CD34
+
subpopulations by sequential sorting. CD34
+
hematopoietic stem and progenitor cells can be isolated from
  • peripheral blood,
  • bone marrow aspirate,
  • leukapheresis product,
  • cord blood,
  • differentiated ES and iPS cell cultures1.

Detailed product information

Detailed separation procedure

Using the CD34 MultiSort Kit, in the first step CD34
+
cells are positively selected after labeling with the CD34 MultiSort MicroBeads. Subsequently, the MultiSort MicroBeads are enzymatically released from the CD34 antibody. The isolated CD34
+
cells can be magnetically labeled and sorted according to a second antigen. The kit contains CD34 MultiSort MicroBeads, MultiSort Release Reagent, and MultiSort Stop Reagent.
Control staining after separation requires a monoclonal CD34 antibody other than QBEND/10, e.g. clone AC136

Downstream applications

The CD34 MultiSort Kit is used to isolate specific subsets of CD34
+
hematopoietic stem and progenitor cells. CD34-selected cells can be further sorted according to their expression of, e.g., HLA-DR, CD43, CD45RA, CD61, CD71, CD90 (Thy1), or CD133.

Columns

MS, LS, XS, or autoMACS
®
Columns.

References for CD34 MultiSort Kit, human

Publications

  1. Vodoyanik, M. A. et al. (2006) Leukosialin (CD43) defines hematopoietic progenitors in human embryonic stem cell differentiation cultures. Blood 108: 2095-2105

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