The CD34
+
CD38
Cell Isolation Kit, human has been developed to isolate a CD38
subset of CD34
+
cells, i.e., primitive hematopoietic stem and progenitor cells, from cord blood, bone marrow, or apheresis.

Data and images for
CD34
+
CD38
-
Cell Isolation Kit
, human

Figures

Figure 1

CD34
+
CD38
cells were isolated from human cord blood MNCs by using the CD34 MultiSort MicroBeads, two LS Columns, and a MidiMACS™ Separator for the first separation. In the second separation step, the CD38 MicroBeads, one LS Column, and a MidiMACS Separator have been used. Cells were fluorescently stained with CD34-FITC, CD38-APC, CD45-VioBlue
®
, Labeling Check Reagent‑APC, and Propidium Iodide Solution and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Before separation
Before CD38 depletion
View details

Figure 1

CD34
+
CD38
cells were isolated from human cord blood MNCs by using the CD34 MultiSort MicroBeads, two LS Columns, and a MidiMACS™ Separator for the first separation. In the second separation step, the CD38 MicroBeads, one LS Column, and a MidiMACS Separator have been used. Cells were fluorescently stained with CD34-FITC, CD38-APC, CD45-VioBlue
®
, Labeling Check Reagent‑APC, and Propidium Iodide Solution and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

CD34
+
CD38
cells were isolated from human cord blood MNCs by using the CD34 MultiSort MicroBeads, two LS Columns, and a MidiMACS™ Separator for the first separation. In the second separation step, the CD38 MicroBeads, one LS Column, and a MidiMACS Separator have been used. Cells were fluorescently stained with CD34-FITC, CD38-APC, CD45-VioBlue
®
, Labeling Check Reagent‑APC, and Propidium Iodide Solution and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
CD34
+
CD38
cells
CD34
+
CD38
+
cells
View details

Figure 1

CD34
+
CD38
cells were isolated from human cord blood MNCs by using the CD34 MultiSort MicroBeads, two LS Columns, and a MidiMACS™ Separator for the first separation. In the second separation step, the CD38 MicroBeads, one LS Column, and a MidiMACS Separator have been used. Cells were fluorescently stained with CD34-FITC, CD38-APC, CD45-VioBlue
®
, Labeling Check Reagent‑APC, and Propidium Iodide Solution and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

CD34
+
CD38
cells were isolated from human cord blood MNCs by using the CD34 MultiSort MicroBeads, two LS Columns, and a MidiMACS™ Separator for the first separation. In the second separation step, the CD38 MicroBeads, one LS Column, and a MidiMACS Separator have been used. Cells were fluorescently stained with CD34-FITC, CD38-APC, CD45-VioBlue
®
, Labeling Check Reagent‑APC, and Propidium Iodide Solution and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for
CD34
+
CD38
-
Cell Isolation Kit
, human

Overview

The CD34
+
CD38
Cell Isolation Kit, human has been developed to isolate a CD38
subset of CD34
+
cells, i.e., primitive hematopoietic stem and progenitor cells, from cord blood, bone marrow, or apheresis.

Detailed product information

Background information

The CD34
+
CD38
Cell Isolation Kit has been developed for the isolation of the more primitive CD38
subpopulation of CD34
+
cells. CD34 is a well-established marker of human hematopoietic stem and progenitor cells and additionally expressed on hemangioblasts, endothelial progenitor cells, and mature endothelial cells. CD38 is absent on most primitive CD34
+
stem cells and upregulated on CD34
+
cells upon lymphocyte commitment. CD34
+
CD38
-
cells are isolated in a two-step isolation protocol. First, the CD34
+
cells are positively enriched using CD34 MultiSort MicroBeads. Subsequently, the MultiSort MicroBeads are enzymatically released from the CD34 antibody. The isolated CD34
+
are then labeled with CD38 MicroBeads to deplete CD38
+
cells in a second separation step.

Downstream applications

  • CD34+ CD38 cell expansion in culture
  • Lentiviral transduction
  • Molecular analysis
  • In vitro differentiation of CD34+ CD38 cells
  • Generation of humanized mice

Columns

LS Columns.

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CD34
+
CD38
-
Cell Isolation Kit
, human

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