Clone:
FR3-16A11
Type of antibody:
Primary antibodies
Isotype:
mouse IgG1κ
Alternative names:
ENPP3, B10, NPP3, PD-IBETA, PDNP3, E-NPP3, PD-1b

Specifications for CD203c antibodies

Overview

Clone FR3-16A11 recognizes CD203c, a glycosylated type II transmembrane molecule that belongs to the family of ecto-nucleotide pyrophosphatase/ phosphodiesterase (E-NPP3) enzymes. Among hematopoietic cells, expression of CD203c is restricted to basophils as well as to mast cells and their precursors, and has been described as specific for this lineage. Protein and/or mRNA expression of CD203c has also been found in solid tissues such as uterus or prostate. Basophils and mast cells are key producers of mediators that drive the onset of inflammatory responses, e.g., in allergy. Allergen challenge leads to a rapid up-regulation of activation markers such as CD203c or CD63. Due to its restricted expression pattern, CD203c is discussed as a specific marker to monitor the allergen-induced activation of basophils, e.g., in flow cytometric basophil activation tests of the peripheral blood.

Applications

Reagent can be used for immunophenotyping by flow cytometry. Abnormal numbers of cells expressing this antigen or aberrant expression levels of the antigen can be expected in some disease states. It is important to understand the normal expression pattern for this antigen and its relationship to expression of other relevant antigens in order to perform appropriate analysis.
Expression of CD203c may be used as aid to diagnostic in the characterization of samples from individuals suspected with hematologic neoplasia.

Alternative names

ENPP3, B10, NPP3, PD-IBETA, PDNP3, E-NPP3, PD-1b

Detailed product information

Technical specifications

CloneFR3-16A11
Isotypemouse IgG1κ
Type of antibodyPrimary antibodies
Specieshuman
AntigenCD203c
Alternative names of antigenENPP3, B10, NPP3, PD-IBETA, PDNP3, E-NPP3, PD-1b
Molecular mass of antigen [kDa]100
Distribution of antigenmast cells, myeloid cells, basophils

Resources for CD203c antibodies

Certificates

Please follow this
link
to download the Certificate of Conformity (CoC) by lot number.

References for CD203c antibodies

Publications

  1. Bühring, H. J. et al. (1999) The monoclonal antibody 97A6 defines a novel surface antigen expressed on human basophils and their multipotent and unipotent progenitors. Blood 94: 2343-2356
  2. Goding, J. W. (2000) Ecto-enzymes: physiology meets pathology. J. Leukoc. Biol. 67: 285-311
  3. Kahlert, H. et al. (2003) Measurement of basophil-activating capacity of grass pollen allergens, allergoids Clin. Exp. Allergy 33: 1266-1272
  4. Stelzer GT et al. (1997) U.S.-Canadian consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry: standardization and validation of laboratory procedures. Cytometry 30(5): 214-230
  5. Bühring, H. J. et al. (2004) The basophil-specific ectoenzyme E-NPP3 (CD203c) as a marker for cell activation and allergy diagnosis. Int. Arch. Allergy Immunol. 133: 317-329
  6. Hauswirth, A. W. et al. (2002) Recombinant allergens promote expression of CD203c on basophils in sensitized individuals. J. Allergy Clin. Immunol. 110: 102-109
  7. Rothe, G et al. (2012) Consensus protocol for the flow cytometric Immunophenotyping of hematopoietic malignancies. Leukemia 10(5): 877-895
  8. van Dongen, J. J. M. et al. (2012) EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia 26(9): 1908-1975
  9. Clinical and Laboratory Standards Institute (CLSI) (2007) Clinical Flow Cytometric Analysis of Neoplastic Hematolympoid Cells, CLSI document H43-A2 (ISBN 1-56238-635-2) CLSI; Approved Guideline - Second Edition
  10. Clinical and Laboratory Standards Institute (CLSI) (2007) Enumeration of immunologically defined cell populations by flow cytometry, CLSI document H42-A2 (ISBN 1-56238-640-9) CLSI; Approved Guideline - Second Edition

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