Clone:
REA543
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC, MICS, IF, IHC

Specifications for CD20 Cytoplasmic Antibody, anti-human, REAfinity™

Overview

Clone REA543 recognizes the cytoplasmic domain of the human CD20 antigen, a non-glycosylated transmembrane protein of 33–37 kDa that is expressed on B lineage cells from the pre–B cell stage to the B cell lymphoblast stage. The antigen is further expressed on most malignant B cells. CD20 is not found on early B cell progenitors or plasma cells. Oligomers of CD20 form a Ca
2+
channel and might function in the regulation of local responses during B cell activation.
In vitro
effects of CD20-specific antibodies on resting B cells indicate that CD20 is able to transduce an extracellular signal affecting the G0/G1 cell cycle transition. Studies have demonstrated that CD20-initiated intracellular signals involve tyrosine kinase activation and that CD20 is tightly associated with both serine and tyrosine kinases.
Additional information: Clone REA543 displays negligible binding to Fc receptors.

Detailed product information

Technical specifications

CloneREA543
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (I), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD20 Cytoplasmic
Molecular mass of antigen [kDa]33
Distribution of antigenB cells
Entrez Gene ID931
RRIDAB_2656085, AB_2656086, AB_2656087, AB_2656088, AB_2656089, AB_2656090, AB_2656091, AB_2656092

Reviews for CD20 Cytoplasmic Antibody, anti-human, REAfinity™

Human Anti-CD20-APC-Vio770 Antibody

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CD20 Cytoplasmic-APC-Vio770, human (130-108-292)

This anti-CD20 antibody was tested in order to establish a panel to describe and distinguish peripheral blood lymphocytes (from human PBMCs). We are particularly interested in the innate compartment of lymphocytes, and use bright CD20 and CD19 recognizing antibodies to identify and exclude B lymphocytes. The Anti-CD20-APC-Vio770 human antibody from Miltenyi Biotec is a mouse monoclonal antibody to LT20 to CD20.

References for CD20 Cytoplasmic Antibody, anti-human, REAfinity™

Publications

  1. Einfeld, D. A. et al. (1988) Molecular cloning of the human B cell CD20 receptor predicts a hydrophobic protein with multiple transmembrane domains. EMBO J. 7(3): 711-717
  2. Deans, J. P. et al. (1995) Association of 75/80-kDa phosphoproteins and the tyrosine kinases Lyn, Fyn, and Lck with the B cell molecule CD20. Evidence against involvement of the cytoplasmic regions of CD20. J. Biol. Chem. 270(38): 22632-22638
  3. Vaughan, A. T. et al. (2015) Activatory and inhibitory Fcγ receptors augment rituximab-mediated internalization of CD20 independent of signaling via the cytoplasmic domain. J. Biol. Chem. 290(9): 5424-5437

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