Clone:
REA108
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MC
Alternative names:
CCR7, BLR-2, CC-CKR-7, CCR-7, Cdw197, CMKBR7, EBI-1

Extended validation for CD197 (CCR7) Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA108
3D12+
TG8++
150503++
G043H7+
REA546+
FR11-11E8++
Cells were incubated with an excess of purified unconjugated CD197 (CCR7) (REA108) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD197 (CCR7). Human peripheral blood mononuclear cells (PBMCs) were stained with CD197 (CCR7) antibodies and with a suitable counterstaining. As a control, CD197 (CCR7) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD197 (CCR7). Human peripheral blood mononuclear cells (PBMCs) were stained with CD197 (CCR7) antibodies and with a suitable counterstaining. As a control, CD197 (CCR7) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD197 (CCR7). Human peripheral blood mononuclear cells (PBMCs) were stained with CD197 (CCR7) antibodies and with a suitable counterstaining. As a control, CD197 (CCR7) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD197 (CCR7). Human peripheral blood mononuclear cells (PBMCs) were stained with CD197 (CCR7) antibodies and with a suitable counterstaining. As a control, CD197 (CCR7) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD197 (CCR7). Human peripheral blood mononuclear cells (PBMCs) were stained with CD197 (CCR7) antibodies and with a suitable counterstaining. As a control, CD197 (CCR7) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD197 (CCR7). Human peripheral blood mononuclear cells (PBMCs) were stained with CD197 (CCR7) antibodies and with a suitable counterstaining. As a control, CD197 (CCR7) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD197 (CCR7). Human peripheral blood mononuclear cells (PBMCs) were stained with CD197 (CCR7) antibodies and with a suitable counterstaining. As a control, CD197 (CCR7) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD197 (CCR7). Human peripheral blood mononuclear cells (PBMCs) were stained with CD197 (CCR7) antibodies and with a suitable counterstaining. As a control, CD197 (CCR7) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD197 (CCR7) (REA108). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD197 (CCR7) (REA108). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD197 (CCR7) (REA108). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD197 (CCR7) Antibody, anti-human, REAfinity™

Overview

REA108 recognizes human CD197 which is a chemokine receptor with a C-C motif that mediates homing of T cells to secondary lymphoid organs via high endothelial venules (HEV) and is also known as CCR7. For example, naive T cells migrate very efficiently through lymph nodes using the adhesion molecules L-selectin, CD62L, and CCR7. Ligands for CCR7, e.g. CCL19, are expressed by the HEV of secondary lymphoid organs, by parenchymal cells within T cell zones of lymph nodes, and by endothelial cells at the openings of lymphatic vessels within peripheral tissues. Expression of CCR7 and the CD45RA isoform distinguishes three subsets of T cells: naive T cells (CCR7
+
CD45RA
+
), central memory T cells (CCR7
+
CD45RA
), and effector memory T cells (CCR7
CD45RA
).
Additional information: Clone REA108 displays negligible binding to Fc receptors.

Alternative names

CCR7, BLR-2, CC-CKR-7, CCR-7, Cdw197, CMKBR7, EBI-1

Detailed product information

Technical specifications

CloneREA108
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD197 (CCR7)
Alternative names of antigenCCR7, BLR-2, CC-CKR-7, CCR-7, Cdw197, CMKBR7, EBI-1
Molecular mass of antigen [kDa]40
Distribution of antigenB cells, dendritic cells, NK cells, macrophages, thymocytes
Entrez Gene ID1236
RRIDAB_2751410, AB_2733212, AB_2733213, AB_2784046, AB_2784045, AB_2784044, AB_2784043, AB_2752158, AB_2752107, AB_2751042, AB_2751428

Resources for CD197 (CCR7) Antibody, anti-human, REAfinity™

References for CD197 (CCR7) Antibody, anti-human, REAfinity™

Publications

  1. Bacher, P. et al. (2014)
    Identification of immunogenic antigens from
    Aspergillus fumigatus
    by direct multi parameter characterization of specific conventional and regulatory CD4
    +
    T cells.
    J. Immunol. 193: 3332-3343
  2. Sallusto, F. et al. (1999) Two subsets of memory T lymphocytes with distinct homing potentials and effector functions. Nature 401: 708-712
  3. Baldan, W. et al. (2015) Efficient and reproducible generation of tumour-infiltrating lymphocytes for renal cell carcinoma. Br. J. Cancer 112(9): 1510-1518
  4. Frascaroli, G. et al. (2018)
    Human macrophages escape inhibition of major histocompatibility complex-dependent antigen presentation by
    Cytomegalovirus
    and drive proliferation and activation of memory CD4
    +
    and CD8
    +
    T cells.
    Front Immunol 9
  5. Tilly, G. et al. (2017) IL-15 Harnesses Pro-inflammatory Function of TEMRA CD8 in Kidney-Transplant Recipients. Front Immunol 8
  6. Alvarez-Fernández, C. et al. (2016) A short CD3/CD28 costimulation combined with IL-21 enhance the generation of human memory stem T cells for adoptive immunotherapy. J. Transl. Med. 14: 214
  7. Chang, Z. L. et al. (2015) Identification and selective expansion of functionally superior T cells expressing chimeric antigen receptors. J. Transl. Med. 13: 161

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