Clone:
REA538
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IHC, IF
Alternative names:
CCR2, CC-CKR-2, CCR2B, Ckr2, CKR2A, Ckr2b, Cmkbr2, mJe-r, MCP-1-R

Extended validation for CD192 (CCR2) Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA538
475301++
SA203G11-
Cells were incubated with an excess of purified unconjugated CD192 (CCR2) (REA538) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD192 (CCR2). Bone marrow cells from C57BL/6 mice were stained with CD192 (CCR2) antibodies and with a suitable counterstaining. As a control, CD192 (CCR2) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD192 (CCR2). Bone marrow cells from C57BL/6 mice were stained with CD192 (CCR2) antibodies and with a suitable counterstaining. As a control, CD192 (CCR2) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD192 (CCR2). Bone marrow cells from C57BL/6 mice were stained with CD192 (CCR2) antibodies and with a suitable counterstaining. As a control, CD192 (CCR2) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD192 (CCR2). Bone marrow cells from C57BL/6 mice were stained with CD192 (CCR2) antibodies and with a suitable counterstaining. As a control, CD192 (CCR2) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD192 (CCR2) (REA538). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD192 (CCR2) (REA538). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD192 (CCR2) (REA538). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD192 (CCR2) Antibody, anti-mouse, REAfinity™

Overview

Clone REA538 recognizes the mouse CD192 antigen, a multi-pass membrane protein, which is also known as C-C chemokine receptor type 2 (CCR2) or monocyte chemoattractant protein 1 receptor (MCP-1-R). CD192 is a receptor for the CCL2, CCL7, and CCL12 chemokines and plays important roles in the recruitment of monocytes, macrophages, and T cells. It transduces a signal by increasing intracellular calcium ion levels. In mice CD192 is homogeneously expressed on monocytes and on 2–15% of T cells, closely resembling the expression pattern in humans. The percentage of CD192
+
T cells is almost two times higher in the Tʜ1-prone mouse strain C57BL/6 than in the Tʜ2-prone strain BALB/c. The strain-dependent expression correlates to a reduced Tʜ1 response described in CD192-deficient mice.
Additional information: Clone REA538 displays negligible binding to Fc receptors.

Alternative names

CCR2, CC-CKR-2, CCR2B, Ckr2, CKR2A, Ckr2b, Cmkbr2, mJe-r, MCP-1-R

Detailed product information

Technical specifications

CloneREA538
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenCD192 (CCR2)
Alternative names of antigenCCR2, CC-CKR-2, CCR2B, Ckr2, CKR2A, Ckr2b, Cmkbr2, mJe-r, MCP-1-R
Molecular mass of antigen [kDa]43
Distribution of antigenmacrophages, monocytes, T cells
Entrez Gene ID12772
RRIDAB_2876952, AB_2751785, AB_2784036, AB_2655862, AB_2655863, AB_2727981

References for CD192 (CCR2) Antibody, anti-mouse, REAfinity™

Publications

  1. Mack, M. et al. (2001) Expression and characterization of the chemokine receptors CCR2 and CCR5 in mice. J. Immunol. 166(7): 4697-4704
  2. Majmudar, M. D. et al. (2013) Monocyte-directed RNAi targeting CCR2 improves infarct healing in atherosclerosis-prone mice. Circulation 127(20): 2038-2046
  3. Kurihara, T. et al. (1996) Cloning and functional expression of mCCR2, a murine receptor for the C-C chemokines JE and FIC. J. Biol. Chem. 271(20): 11603-11607

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