Clone:
6D5
Type of antibody:
Primary antibodies
Isotype:
rat IgG2aκ
Applications:
FC, MICS, IF, IHC
Alternative names:
B4

Extended validation for CD19 Antibody, anti-mouse

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with 6D5
REA749++
1D3++
MB19-1++
Cells were incubated with an excess of purified unconjugated CD19 (6D5) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD19. C57BL6/6 splenocytes were stained with CD19 antibodies and with a suitable counterstaining. As a control, CD19 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD19. C57BL6/6 splenocytes were stained with CD19 antibodies and with a suitable counterstaining. As a control, CD19 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD19. C57BL6/6 splenocytes were stained with CD19 antibodies and with a suitable counterstaining. As a control, CD19 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD19. C57BL6/6 splenocytes were stained with CD19 antibodies and with a suitable counterstaining. As a control, CD19 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD19. C57BL6/6 splenocytes were stained with CD19 antibodies and with a suitable counterstaining. As a control, CD19 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD19. C57BL6/6 splenocytes were stained with CD19 antibodies and with a suitable counterstaining. As a control, CD19 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD19. C57BL6/6 splenocytes were stained with CD19 antibodies and with a suitable counterstaining. As a control, CD19 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD19. C57BL6/6 splenocytes were stained with CD19 antibodies and with a suitable counterstaining. As a control, CD19 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD19 (6D5). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD19 (6D5). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD19 (6D5). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD19 Antibody, anti-mouse

Overview

Clone 6D5 recognizes the mouse CD19 antigen, a type I transmembrane glycoprotein expressed on B cells throughout their development from the early pro–B cell through the mature B cell stages. Its expression is down-regulated during terminal differentiation to plasma cells. CD19 associates with complement receptor CD21 and with CD81 on the cell surface of mature B cells, thereby forming a multi-molecular complex which signals synergistically to membrane IgM. It is also expressed on follicular dendritic cells and peritoneal mast cells.

Alternative names

B4

Detailed product information

Technical specifications

Clone6D5
Clonalitymonoclonal
Isotyperat IgG2aκ
Isotype controlIsotype Control Antibody, rat IgG2a
Hostrat
Type of antibodyPrimary antibodies
Speciesmouse
AntigenCD19
Alternative names of antigenB4
Molecular mass of antigen [kDa]58
Distribution of antigenB cells, dendritic cells
Entrez Gene ID12478
RRIDAB_2751519, AB_2801733, AB_2801732, AB_2819465, AB_2857605, AB_2811554, AB_2811568, AB_2661121, AB_2751853

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