Clone:
REA724
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
C-X-C chemokine receptor type 3, CXC-R3, CXCR-3, Interferon-inducible protein 10 receptor, IP-10 receptor

Extended validation for CD183 (CXCR3) Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA724
220803++
CXCR3-173++
S18001A++
Cells were incubated with an excess of purified unconjugated CD183 (CXCR3) (REA724) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

View details
Flow cytometric comparison of different clones for CD183 (CXCR3). C57BL6/6 splenocytes were stained with CD183 (CXCR3) antibodies and with a suitable counterstaining. As a control, CD183 (CXCR3) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD183 (CXCR3). C57BL6/6 splenocytes were stained with CD183 (CXCR3) antibodies and with a suitable counterstaining. As a control, CD183 (CXCR3) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD183 (CXCR3). C57BL6/6 splenocytes were stained with CD183 (CXCR3) antibodies and with a suitable counterstaining. As a control, CD183 (CXCR3) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD183 (CXCR3). C57BL6/6 splenocytes were stained with CD183 (CXCR3) antibodies and with a suitable counterstaining. As a control, CD183 (CXCR3) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD183 (CXCR3). C57BL6/6 splenocytes were stained with CD183 (CXCR3) antibodies and with a suitable counterstaining. As a control, CD183 (CXCR3) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD183 (CXCR3) (REA724). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD183 (CXCR3) (REA724). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD183 (CXCR3) (REA724). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD183 (CXCR3) Antibody, anti-mouse, REAfinity™

Overview

Clone REA724 recognizes the mouse CD183 antigen, also known as CXCR3, a 40 kDa seven-transmembrane protein belonging to the the G protein–coupled receptor family that mediates the proliferation, survival, and angiogenic activity of mesangial cells through a heterotrimeric G protein signaling pathway. CD183 binds the ligands CXCL9, CXCL10, and CXCL11 and mediates Ca
2+
mobilization and chemotaxis. It is expressed on activated T cells, circulating blood T cells, B cells, and NK cells.
Additional information: Clone REA724 displays negligible binding to Fc receptors.

Alternative names

C-X-C chemokine receptor type 3, CXC-R3, CXCR-3, Interferon-inducible protein 10 receptor, IP-10 receptor

Detailed product information

Technical specifications

CloneREA724
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenCD183 (CXCR3)
Alternative names of antigenC-X-C chemokine receptor type 3, CXC-R3, CXCR-3, Interferon-inducible protein 10 receptor, IP-10 receptor
Molecular mass of antigen [kDa]41
Distribution of antigenT cells, NK cells, B cells
Entrez Gene ID12766
RRIDAB_2655745, AB_2655746, AB_2655747, AB_2655748, AB_2655749, AB_2655750, AB_2655751, AB_2655744

References for CD183 (CXCR3) Antibody, anti-mouse, REAfinity™

Publications

  1. Tamaru, M. et al. (1998) Cloning of the murine interferon-inducible protein 10 (IP-10) receptor and its specific expression in lymphoid organs. Biochem. Biophys. Res. Commun. 251(1): 41-48
  2. Soto, H. et al. (1998) The CC chemokine 6Ckine binds the CXC chemokine receptor CXCR3. Proc. Natl. Acad. Sci. U.S.A. 95(14): 8205-8210
  3. Huttlin, E. L. et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143(7): 1174-1189

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