Clone:
REA258
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
MC, FC, MICS, IF, IHC, ICC
Alternative names:
HNA-2a, NB1, NB1 GP, PRV-1, PRV1

Specifications for CD177 Antibody, anti-human, REAfinity™

Overview

Clone REA258 recognizes CD177, which is also known as neutrophil specific antigen 1. CD177, a member of the µPAR family, is a 58–64 kDa glycosylphosphatidylinositol-anchored glycoprotein expressed exclusively by subset of neutrophils, neutrophilic metamyelocytes, and myelocytes and is suggested to play a role in neutrophil mediated immune reponse as evident by its up-regulation on neutrophils in a number of inflammatory settings such as bacterial infection. Furthermore, CD177 can directly bind to PECAM-1 allowing neutrophil-endothelial cell interactions and thus contributing to neutrophil transmigration. Overexpression of CD177 is also seen in patients with polycythemia vera, making it a useful diagnostic marker.
Additional information: Clone REA258 displays negligible binding to Fc receptors.

Alternative names

HNA-2a, NB1, NB1 GP, PRV-1, PRV1

Detailed product information

Technical specifications

CloneREA258
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, non-human primate
Cross-reactivity
rhesus monkey (
Macaca mulatta
)
AntigenCD177
Alternative names of antigenHNA-2a, NB1, NB1 GP, PRV-1, PRV1
Molecular mass of antigen [kDa]41
Distribution of antigengranulocytes, neutrophils
Entrez Gene ID57126
RRIDAB_2655641, AB_2655642, AB_2655643, AB_2655644, AB_2655645, AB_2655646, AB_2655647, AB_2655648, AB_2655649, AB_2655650

Reviews for CD177 Antibody, anti-human, REAfinity™

Miltenyi CD177 on Human Peripheral Blood Neutrophils

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CD177-PE, human (130-101-523)

The CD177 antibody by Miltenyi Biotec worked like a charm for us. It worked without large titrations and blockings on our human peripheral blood neutrophils and we will be able to go on with our experiments sooner than expected.

Miltenyi CD177 on Human Peripheral Blood Neutrophils

  • 1
  • 2
  • 3
  • 4
  • 5

CD177-PE, human (130-101-525)

The CD177 antibody by Miltenyi Biotec worked like a charm for us. It worked without large titrations and blockings on our human peripheral blood neutrophils and we will be able to go on with our experiments sooner than expected.

References for CD177 Antibody, anti-human, REAfinity™

Publications

  1. Sachs, U. J. H. et al. (2007) The neutrophil-specific antigen CD177 is a counter-receptor for platelet endothelial cell adhesion molecule-1 (CD31). J. Biol. Chem. 282: 23603 -23612
  2. Monti, M. et al. (2017) Integrin-dependent cell adhesion to neutrophil extracellular traps through engagement of fibronectin in neutrophil-like cells. PLoS One 12
  3. Beyrau, M. et al. (2012) Neutrophil heterogeneity in health and disease: a revitalized avenue in inflammation and immunity. Open Bio 2: 120134
  4. Wu, Z. et al. (2016) Heterogeneity of human neutrophil CD177 expression results from CD177P1 pseudogene conversion. PLoS Genet. 12(5)
  5. Tackeray, J. T. et al. (2016) Targeting amino acid metabolism for molecular imaging of inflammation early after myocardial infarction. Theranostics 6(11): 1768-1779

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