CD16 MicroBeads have been developed for the positive selection or depletion of NK cells from rhesus monkey PBMCs. CD16 is expressed on the majority of rhesus monkey NK cells
1
and on a subset of monocytes. Monocytes can be depleted prior to separation using CD56 MicroBeads or CD14 MicroBeads, respectively.

Data and images for CD16 MicroBeads, non-human primate

Figures

Figure 1

Positive selection of CD16
+
NK cells from rhesus monkey PBMCs using CD16 MicroBeads, a MiniMACS™ Separator, and an MS Column. As some rhesus monkey monocytes express CD16, monocytes1 as well as a minor subset of CD56
+
NK cells2 were depleted by using CD56 MicroBeads prior to positive selection of CD16
+
cells.
PBMCs after depletion of CD56
+
cells
CD16
+
NK cells
View details

Figure 1

Positive selection of CD16
+
NK cells from rhesus monkey PBMCs using CD16 MicroBeads, a MiniMACS™ Separator, and an MS Column. As some rhesus monkey monocytes express CD16, monocytes1 as well as a minor subset of CD56
+
NK cells2 were depleted by using CD56 MicroBeads prior to positive selection of CD16
+
cells.
View details

Figure 1

Positive selection of CD16
+
NK cells from rhesus monkey PBMCs using CD16 MicroBeads, a MiniMACS™ Separator, and an MS Column. As some rhesus monkey monocytes express CD16, monocytes1 as well as a minor subset of CD56
+
NK cells2 were depleted by using CD56 MicroBeads prior to positive selection of CD16
+
cells.

Specifications for CD16 MicroBeads, non-human primate

Overview

CD16 MicroBeads have been developed for the positive selection or depletion of NK cells from rhesus monkey PBMCs. CD16 is expressed on the majority of rhesus monkey NK cells
1
and on a subset of monocytes. Monocytes can be depleted prior to separation using CD56 MicroBeads or CD14 MicroBeads, respectively.

Detailed product information

Downstream applications

CD16
+
NK cells can, for example, be used for studies on NK cell cytotoxicity or signal transduction.

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.

References for CD16 MicroBeads, non-human primate

Publications

  1. Carter, D. L. et al. (1999)
    CD56 identifies monocytes and not natural killer cells in
    Rhesus macaques
    .
    Cytometry 37: 41-50
  2. Webster, R. L. and Johnson, R. P. (2005) Delineation of multiple subpopulations of natural killer cells in rhesus macaques. Immunology 115: 206-214

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