Clone:
REAL107
Type of antibody:
Releasable antibodies, Primary antibodies, Recombinant antibodies
Applications:
FC, MICS, IF, IHC
Alternative names:
LPS R

Specifications for CD14 Antibody, anti-human,
REAlease
®

Overview

Clone REAL107 is an antibody fragment derived from the full CD14 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.
Clone REAL107 recognizes the human CD14 antigen. CD14 is part of the functional heteromeric LPS receptor complex comprised of at least CD14, TLR4, and MD-2. It up-regulates cell surface molecules, including adhesion molecules. CD14 is strongly expressed on most human monocytes and macrophages in peripheral blood, other bodily fluids, and in various tissues such as lymph nodes and spleen. CD14 is weakly expressed on subpopulations of human neutrophils and myeloid dendritic cells.
The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

LPS R

Detailed product information

Technical specifications

CloneREAL107
Clonalitymonoclonal
Isotype controlControl Antibody
Hostcell line
Type of antibodyReleasable antibodies, Primary antibodies, Recombinant antibodies
Specieshuman
AntigenCD14
Alternative names of antigenLPS R
Distribution of antigenmonocytes, macrophages
RRIDAB_2751068, AB_2751811, AB_2751754, AB_2783951, AB_2783950, AB_2811693, AB_2751092

References for CD14 Antibody, anti-human,
REAlease
®

Publications

  1. Goyer, S. M. and Ferrero, E. (1987) Biochemical analysis of myeloid antigen and cDNA expression of gp55 (CD14). Oxford, Oxford University Press
  2. Wilson, A. D. et al. (1995)
    Selection of monoclonal antibodies for the identification of lymphocyte surface antigens in the New World primate
    Saguinus oedipus oedipus
    (cotton top tamarin).
    J. Immunol. Methods 178: 195-200
  3. Dzionek, A. et al. (2000) BDCA-2, BDCA-3, BDCA-4: Three markers for distinct subsets of dendritic cells in human peripheral blood. J. Immunol. 165: 6037-6046
  4. Neu, C. et al. (2013) CD14-dependent monocyte isolation enhances phagocytosis of listeria monocytogenes by proinflammatory, GM-CSF-derived macrophages. PLoS One 8(6): e66898
  5. Paccou, J. et al. (2013)
    Determination and modulation of total and surface calcium-sensing receptor expression in monocytes
    in vivo
    and
    in vitro
    .
    PLoS One 8(10): e74800

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