The CD105 MultiSort Kit (PE), mouse has been developed for the isolation of CD105
+
cells or cell subsets, including
  • vascular endothelial cells from mouse tissue or cell cultures
  • long-term repopulating hematopoietic stem cells (LTR-HSCs) from mouse bone marrow when used in combination with the Anti-Sca-1 MicroBead Kit (FITC).

Data and images for CD105 MultiSort Kit (PE), mouse

Figures

Figure 1

Isolation of CD105
+
Sca-1
+
LTR-HSCs from mouse bone marrow using the CD105 MultiSort Kit (PE), mouse, and two LS Columns for the first positive selection followed by the Anti-Sca-1 MicroBead Kit (FITC), mouse, and two MS Columns in the second positive selection step.
A:
B:
Unseparated fraction
CD105
+
cells
CD105+Sca-1+ LTR-HSCs
View details

Figure 1

Isolation of CD105
+
Sca-1
+
LTR-HSCs from mouse bone marrow using the CD105 MultiSort Kit (PE), mouse, and two LS Columns for the first positive selection followed by the Anti-Sca-1 MicroBead Kit (FITC), mouse, and two MS Columns in the second positive selection step.
View details

Figure 1

Isolation of CD105
+
Sca-1
+
LTR-HSCs from mouse bone marrow using the CD105 MultiSort Kit (PE), mouse, and two LS Columns for the first positive selection followed by the Anti-Sca-1 MicroBead Kit (FITC), mouse, and two MS Columns in the second positive selection step.
View details

Figure 1

Isolation of CD105
+
Sca-1
+
LTR-HSCs from mouse bone marrow using the CD105 MultiSort Kit (PE), mouse, and two LS Columns for the first positive selection followed by the Anti-Sca-1 MicroBead Kit (FITC), mouse, and two MS Columns in the second positive selection step.

Specifications for CD105 MultiSort Kit (PE), mouse

Overview

The CD105 MultiSort Kit (PE), mouse has been developed for the isolation of CD105
+
cells or cell subsets, including
  • vascular endothelial cells from mouse tissue or cell cultures
  • long-term repopulating hematopoietic stem cells (LTR-HSCs) from mouse bone marrow when used in combination with the Anti-Sca-1 MicroBead Kit (FITC).

Detailed product information

Background information

CD105, also known as Endoglin, is abundantly expressed on angiogenic endothelial cells. In mouse bone marrow, CD105 is also expressed on a subpopulation of Sca-1
+
hematopoietic stem cells that displays long-term repopulating activity in lethally irradiated mice (LTR-HSCs)
1
.

Detailed separation procedure

In the first step, CD105
+
cells are positively selected after labeling with the CD105 MultiSort MicroBeads. Subsequently, the MultiSort MicroBeads are enzymatically released from the CD105 antibody. The selected CD105
+
cells can be magnetically labeled and sorted according to a second antigen. The kits contains CD105 MultiSort MicroBeads, MultiSort Release Reagent, and the MultiSort Stop Reagent.

Downstream applications

Isolated CD105
+
Sca-1
+
cells can be transplanted into lethally irradiated mice to evaluate the long-term engraftment potential of this cell population.

Columns

MS, LS, or autoMACS
®
Columns.

Resources for CD105 MultiSort Kit (PE), mouse

References for CD105 MultiSort Kit (PE), mouse

Publications

  1. Chen, C. Z. et al. (2002) Identification of endoglin as a functional marker that defines long-term repopulating hematopoietic stem cells. Proc. Natl. Acad. Sci. U.S.A. 99: 15468-15473
  2. Chen, C. Z. et al. (2003) The endoglin(positive) sca-1(positive) rhodamine(low) phenotype defines a near-homogeneous population of long-term repopulating hematopoietic stem cells. Immunity 19: 525-533

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