Clone:
REA236
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
Itgb4

Extended validation for CD104 (Integrin β4) Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA236
439-9B++
58XB4-
450-9D mIgG1+
422325 mIgG2b-
REAL151++
Cells were incubated with an excess of purified unconjugated CD104 (Integrin β4) (REA236) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD104 (Integrin β4). HT-29 cells were stained with CD104 (Integrin β4) antibodies and plotted against the side scatter. As a control, CD104 (Integrin β4) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD104 (Integrin β4). HT-29 cells were stained with CD104 (Integrin β4) antibodies and plotted against the side scatter. As a control, CD104 (Integrin β4) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD104 (Integrin β4). HT-29 cells were stained with CD104 (Integrin β4) antibodies and plotted against the side scatter. As a control, CD104 (Integrin β4) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD104 (Integrin β4). HT-29 cells were stained with CD104 (Integrin β4) antibodies and plotted against the side scatter. As a control, CD104 (Integrin β4) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD104 (Integrin β4). HT-29 cells were stained with CD104 (Integrin β4) antibodies and plotted against the side scatter. As a control, CD104 (Integrin β4) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD104 (Integrin β4) (REA236). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD104 (Integrin β4) (REA236). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD104 (Integrin β4) (REA236). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD104 (Integrin β4) Antibody, anti-human, REAfinity™

Overview

Clone REA236 recognizes CD104, which is also known as integrin β4. Integrins are a family of type I transmembrane heterodimeric glycoprotein receptors and exist as non-covalently bound α and β subunits. CD104 interacts with α6 integrin and serves as receptor for laminin and component of hemidesmosomes. Expression of α6β4 integrin is found in the basal aspect of stratified epithelia such as epidermis, stratified squamous epithelium of squamous mucosa, urothelium, and certain tumor cells.
Additional information: Clone REA236 displays negligible binding to Fc receptors.

Alternative names

Itgb4

Detailed product information

Technical specifications

CloneREA236
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD104 (Integrin β4)
Alternative names of antigenItgb4
Distribution of antigenepithelial cells
RRIDAB_2819587, AB_2857656, AB_2857631, AB_2857694, AB_2857682, AB_2857612, AB_2857600, AB_2654407, AB_2654408, AB_2654409, AB_2654410, AB_2654411, AB_2654412, AB_2819602

References for CD104 (Integrin β4) Antibody, anti-human, REAfinity™

Publications

  1. Simon-Assmann, P. et al. (1994) Differential expression of laminin isoforms and alpha 6-beta 4 integrin subunits in the developing human and mouse intestine. Dev. Dyn. 201(1): 71-85
  2. Tuckwell, D. S. et al. (1993) Integrins: a review of their structure and mechanisms of ligand binding. Symp. Soc. Exp. Biol 47: 107-136

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