Name: Basophil Isolation Kit II, human
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Product data and images for Basophil Isolation Kit II, human

Figures

Figure 1

Basophils were enriched from human PBMCs using the Basophil Isolation Kit II, a MidiMACS™ Separator and an LS Column. Cells were stained with CD203c-APC, CD123-PE and CD303 (BDCA-2)-FITC. Basophils were detected as CD203c

⁺

cells or CD123

^bright

CD303

^–

cells because CD123 is expressed at high levels on both basophils and CD303

⁺

plasmacytoid dendritic cells.

Unseparated fraction
A:	B:
View details Figure 1 Basophils were enriched from human PBMCs using the Basophil Isolation Kit II, a MidiMACS™ Separator and an LS Column. Cells were stained with CD203c-APC, CD123-PE and CD303 (BDCA-2)-FITC. Basophils were detected as CD203c ⁺ cells or CD123 ^bright CD303 ^– cells because CD123 is expressed at high levels on both basophils and CD303 ⁺ plasmacytoid dendritic cells.	View details Figure 1 Basophils were enriched from human PBMCs using the Basophil Isolation Kit II, a MidiMACS™ Separator and an LS Column. Cells were stained with CD203c-APC, CD123-PE and CD303 (BDCA-2)-FITC. Basophils were detected as CD203c ⁺ cells or CD123 ^bright CD303 ^– cells because CD123 is expressed at high levels on both basophils and CD303 ⁺ plasmacytoid dendritic cells.
Isolated basophils
A:	B:
View details Figure 1 Basophils were enriched from human PBMCs using the Basophil Isolation Kit II, a MidiMACS™ Separator and an LS Column. Cells were stained with CD203c-APC, CD123-PE and CD303 (BDCA-2)-FITC. Basophils were detected as CD203c ⁺ cells or CD123 ^bright CD303 ^– cells because CD123 is expressed at high levels on both basophils and CD303 ⁺ plasmacytoid dendritic cells.	View details Figure 1 Basophils were enriched from human PBMCs using the Basophil Isolation Kit II, a MidiMACS™ Separator and an LS Column. Cells were stained with CD203c-APC, CD123-PE and CD303 (BDCA-2)-FITC. Basophils were detected as CD203c ⁺ cells or CD123 ^bright CD303 ^– cells because CD123 is expressed at high levels on both basophils and CD303 ⁺ plasmacytoid dendritic cells.

Figure 2

View details

Figure 2

Basophils isolated with MACS

^®

Technology using the Basophil Isolation Kit II. (Courtesy of Dr. Karin Haisch, Borstel, Germany.)

Basophils isolated with MACS

^®

Technology using the Basophil Isolation Kit II. (Courtesy of Dr. Karin Haisch, Borstel, Germany.)

Specifications for Basophil Isolation Kit II, human

Overview

The Basophil Isolation Kit II is an indirect magnetic labeling system for the negative isolation of basophils from PBMCs.

Detailed product information

Detailed separation procedure

Non-basophils (i.e. T cells, NK cells, B cells, monocytes, dendritic cells, erythroid cells, platelets, neutrophils, and eosinophils) are indirectly magnetically labeled using a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. Isolation of highly pure basophils is achieved by depletion of the magnetically labeled cells.

Downstream applications

Isolated basophils have been used for phenotypical characterization

, for histamine, leukotriene, and cytokine

^{1, 4, 5, 6}

release assays, for molecular studies

^{1, 2, 5}

, protein analysis

^{4, 5}

, and other applications.

Columns

LS, XS, or autoMACS

^®

Columns.

References for Basophil Isolation Kit II, human

Publications

Shiratori, I. et al. (2005) Down-regulation of basophil function by human CD200 and human herpesvirus-8 CD200. J. Immunol. 175: 4441-4449
Nishiyama, C. et al. (2004) Polymorphisms in the Fc epsilon RI beta promoter region affecting transcription activity: a possible promoter-dependent mechanism for association between Fc epsilon RI beta and atopy. J. Immunol. 173: 6458-6464
de Paulis, A. et al. (2004) Urokinase induces basophil chemotaxis through a urokinase receptor epitope that is an endogenous ligand for formyl peptide receptor-like 1 and -like 2. J. Immunol. 173: 5739-5748
Gibbs, B. F. et al. (2002) Regulation of mediator secretion in human basophils by p38 mitogen-activated protein kinase: phosphorylation is sensitive to the effects of phosphatidylinositol 3-kinase inhibitors and calcium mobilization. J. Leukoc. Biol. 72: 391-400
Lavens-Phillips, S.E. et al. (2000) The tyrosine kinases p53/56lyn and p72syk are differentially expressed at the protein level but not at the messenger RNA level in nonreleasing human basophils. Am. J. Respir. Cell Mol. Biol. 23: 566-571
Haisch, K. et al. (1999) Purification of morphologically and functionally intact human basophils to near homogeneity. J. Immunol. Methods 226: 129-137

Data and images

Data and images for Basophil Isolation Kit II, human

Figures

Figure 1

⁺

cells or CD123

^bright

CD303

^–

cells because CD123 is expressed at high levels on both basophils and CD303

⁺

plasmacytoid dendritic cells.

Unseparated fraction
A:	B:
View details Figure 1 Basophils were enriched from human PBMCs using the Basophil Isolation Kit II, a MidiMACS™ Separator and an LS Column. Cells were stained with CD203c-APC, CD123-PE and CD303 (BDCA-2)-FITC. Basophils were detected as CD203c ⁺ cells or CD123 ^bright CD303 ^– cells because CD123 is expressed at high levels on both basophils and CD303 ⁺ plasmacytoid dendritic cells.	View details Figure 1 Basophils were enriched from human PBMCs using the Basophil Isolation Kit II, a MidiMACS™ Separator and an LS Column. Cells were stained with CD203c-APC, CD123-PE and CD303 (BDCA-2)-FITC. Basophils were detected as CD203c ⁺ cells or CD123 ^bright CD303 ^– cells because CD123 is expressed at high levels on both basophils and CD303 ⁺ plasmacytoid dendritic cells.
Isolated basophils
A:	B:
View details Figure 1 Basophils were enriched from human PBMCs using the Basophil Isolation Kit II, a MidiMACS™ Separator and an LS Column. Cells were stained with CD203c-APC, CD123-PE and CD303 (BDCA-2)-FITC. Basophils were detected as CD203c ⁺ cells or CD123 ^bright CD303 ^– cells because CD123 is expressed at high levels on both basophils and CD303 ⁺ plasmacytoid dendritic cells.	View details Figure 1 Basophils were enriched from human PBMCs using the Basophil Isolation Kit II, a MidiMACS™ Separator and an LS Column. Cells were stained with CD203c-APC, CD123-PE and CD303 (BDCA-2)-FITC. Basophils were detected as CD203c ⁺ cells or CD123 ^bright CD303 ^– cells because CD123 is expressed at high levels on both basophils and CD303 ⁺ plasmacytoid dendritic cells.

Figure 2

View details

Figure 2

Basophils isolated with MACS

^®

Technology using the Basophil Isolation Kit II. (Courtesy of Dr. Karin Haisch, Borstel, Germany.)

Basophils isolated with MACS

^®

Technology using the Basophil Isolation Kit II. (Courtesy of Dr. Karin Haisch, Borstel, Germany.)

Specifications

Specifications for Basophil Isolation Kit II, human

Overview

The Basophil Isolation Kit II is an indirect magnetic labeling system for the negative isolation of basophils from PBMCs.

Detailed product information

Detailed separation procedure

Downstream applications

Isolated basophils have been used for phenotypical characterization

, for histamine, leukotriene, and cytokine

^{1, 4, 5, 6}

release assays, for molecular studies

^{1, 2, 5}

, protein analysis

^{4, 5}

, and other applications.

Columns

LS, XS, or autoMACS

^®

Columns.

References

References for Basophil Isolation Kit II, human

Publications

Shiratori, I. et al. (2005) Down-regulation of basophil function by human CD200 and human herpesvirus-8 CD200. J. Immunol. 175: 4441-4449
Nishiyama, C. et al. (2004) Polymorphisms in the Fc epsilon RI beta promoter region affecting transcription activity: a possible promoter-dependent mechanism for association between Fc epsilon RI beta and atopy. J. Immunol. 173: 6458-6464
de Paulis, A. et al. (2004) Urokinase induces basophil chemotaxis through a urokinase receptor epitope that is an endogenous ligand for formyl peptide receptor-like 1 and -like 2. J. Immunol. 173: 5739-5748
Gibbs, B. F. et al. (2002) Regulation of mediator secretion in human basophils by p38 mitogen-activated protein kinase: phosphorylation is sensitive to the effects of phosphatidylinositol 3-kinase inhibitors and calcium mobilization. J. Leukoc. Biol. 72: 391-400
Lavens-Phillips, S.E. et al. (2000) The tyrosine kinases p53/56lyn and p72syk are differentially expressed at the protein level but not at the messenger RNA level in nonreleasing human basophils. Am. J. Respir. Cell Mol. Biol. 23: 566-571
Haisch, K. et al. (1999) Purification of morphologically and functionally intact human basophils to near homogeneity. J. Immunol. Methods 226: 129-137

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Basophil Isolation Kit II, human

Basophil Isolation Kit II, human

Product overview

Product data and images for Basophil Isolation Kit II, human

Figures

Figure 1

Figure 1

Figure 1

Figure 1

Figure 1

Figure 2

Figure 2

Specifications for Basophil Isolation Kit II, human

Overview

Detailed product information

Detailed separation procedure

Downstream applications

Columns

References for Basophil Isolation Kit II, human

Publications

Data and images

Data and images for Basophil Isolation Kit II, human

Figures

Figure 1

Figure 1

Figure 1

Figure 1

Figure 1

Figure 2

Figure 2

Specifications

Specifications for Basophil Isolation Kit II, human

Overview

Detailed product information

Detailed separation procedure

Downstream applications

Columns

References

References for Basophil Isolation Kit II, human

Publications

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Basophil Isolation Kit II, human