The direct Anti-TRA-1-60 MicroBeads and the indirect Anti-TRA-1-60 MicroBead Kit have been developed for the positive selection of human pluripotent stem cells based on the expression of the TRA-1-60 antigen.
The direct Anti-TRA-1-60 MicroBeads offer a 10 min isolation procedure, minimizing cell handling time.

Data and images for Anti-TRA-1-60 MicroBeads, human

Figures

Figure 1

Pluripotent (TRA-1-60
+
) iPSCs were isolated from cultures containing spontaneously differentiated iPSCs grown on mouse embryonic feeder cells using Anti-TRA-1-60 MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-TRA-1-60-PE (# 130-100-347) and Anti-Feeder-APC after separation and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Unseparated fraction
Enriched TRA-1-60
+
pluripotent stem cells
View details

Figure 1

Pluripotent (TRA-1-60
+
) iPSCs were isolated from cultures containing spontaneously differentiated iPSCs grown on mouse embryonic feeder cells using Anti-TRA-1-60 MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-TRA-1-60-PE (# 130-100-347) and Anti-Feeder-APC after separation and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Pluripotent (TRA-1-60
+
) iPSCs were isolated from cultures containing spontaneously differentiated iPSCs grown on mouse embryonic feeder cells using Anti-TRA-1-60 MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-TRA-1-60-PE (# 130-100-347) and Anti-Feeder-APC after separation and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for Anti-TRA-1-60 MicroBeads, human

Overview

The direct Anti-TRA-1-60 MicroBeads and the indirect Anti-TRA-1-60 MicroBead Kit have been developed for the positive selection of human pluripotent stem cells based on the expression of the TRA-1-60 antigen.
The direct Anti-TRA-1-60 MicroBeads offer a 10 min isolation procedure, minimizing cell handling time.

Detailed product information

Background information

The Anti-TRA-1-60 monoclonal antibody reacts with a pluripotent stem cell–specific antigen expressed on undifferentiated human embryonic stem (ES) cells, induced pluripotent (iPS) cells, embryonal carcinoma (EC) cells, and embryonic germ (EG) cells.
2,3
The expression of TRA-1-60 on human ES cells is down-regulated upon differentiation. The Anti-TRA-1-60 antibody recognizes a neuraminidase-resistant carbohydrate epitope expressed on podocalyxin, a member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein, which has been implicated in the development of aggressiveness in a variety of cancers including breast cancer and prostate cancer.

Applications

  • Positive selection of undifferentiated TRA-1-60+ pluripotent stem cells, for example, human ES and iPS cells.
  • Efficient enrichment of iPS cells during or after reprogramming1,5.

Columns

MS or autoMACS
®
Columns for use with Anti-TRA-1-60 MicroBeads.
LS or autoMACS Columns for use with the Anti-TRA-1-60 MicroBead Kit.

References for Anti-TRA-1-60 MicroBeads, human

Publications

  1. Dick, E. et al. (2011) Faster generation of hiPSCs by coupling high-titer lentivirus and column-based positive selection. Nat. Protoc. 6(6): 701-714
  2. Andrews, P. W. et al. (1984) Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells. Hybridoma 3: 347-361
  3. Chan, E. M. et al. (2009) Live cell imaging distinguishes bona fide human iPS cells from partially reprogrammed cells. Nat. Biotechnol. 27: 1033-1037
  4. Grabundzija, I. et al. (2013) Sleeping Beauty transposon-based system for cellular reprogramming and targeted gene insertion in induced pluripotent stem cells. Nucleic Acids Res. 41(3): 1829-1847
  5. Tanabe, K. et al. (2013) Maturation, not initiation, is the major roadblock during reprogramming toward pluripotency from human fibroblasts. Proc. Natl. Acad. Sci. U.S.A. 110: 12172-12179

Related products for
Anti-TRA-1-60 MicroBeads, human

8 products available | view all