Anti-SSEA-4 MicroBeads have been developed for the positive selection or depletion of SSEA-4
+
pluripotent or tissue stem cells.

Data and images for Anti-SSEA-4 MicroBeads, human

Figures

Figure 1

SSEA-4 expressing cells were isolated from a heterogeneous suspension of U937 cells (SSEA-4
CD326
) and NTERA cells (SSEA-4
+
CD326
+
) using Anti-SSEA-4 MicroBeads, an LS Column, and a MidiMACS Separator. Cells were fluorescently stained with Labeling Check Reagent-FITC and CD326 (EpCAM)-APC. Cell debris and dead cells were excluded based on scatter signals and propidium iodide fluorescence.
Unseparated fraction
After separation
View details

Figure 1

SSEA-4 expressing cells were isolated from a heterogeneous suspension of U937 cells (SSEA-4
CD326
) and NTERA cells (SSEA-4
+
CD326
+
) using Anti-SSEA-4 MicroBeads, an LS Column, and a MidiMACS Separator. Cells were fluorescently stained with Labeling Check Reagent-FITC and CD326 (EpCAM)-APC. Cell debris and dead cells were excluded based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

SSEA-4 expressing cells were isolated from a heterogeneous suspension of U937 cells (SSEA-4
CD326
) and NTERA cells (SSEA-4
+
CD326
+
) using Anti-SSEA-4 MicroBeads, an LS Column, and a MidiMACS Separator. Cells were fluorescently stained with Labeling Check Reagent-FITC and CD326 (EpCAM)-APC. Cell debris and dead cells were excluded based on scatter signals and propidium iodide fluorescence.

Specifications for Anti-SSEA-4 MicroBeads, human

Overview

Anti-SSEA-4 MicroBeads have been developed for the positive selection or depletion of SSEA-4
+
pluripotent or tissue stem cells.

Detailed product information

Background information

Stage-specific embryonic antigen 4 (SSEA-4) is a glycolipid epitope that is found on undifferentiated human embryonic stem (ES) cells
1
, induced pluripotent (iPS) cells
2
embryonal carcinoma (EC) cells
3
, and embryonic germ (EG)
4
cells and a variety of somatic stem cells, such as dental pulp stem cells, umbilical cord blood-derived very small embryonic like stem cells (VSELs)
5
and mesenchymal stromal cells
6
.

Columns

For positive selection: LS or autoMACS
®
Columns. For depletion: LD or autoMACS Columns.

References for Anti-SSEA-4 MicroBeads, human

Publications

  1. Thomson, J. A. et al. (1998) Embryonic stem cell lines derived from human blastocysts. Science 282: 1145-1147
  2. Valamehr, B. et al. (2012) A novel platform to enable the high-throughput derivation and characterization of feeder-free human iPSCs. Sci Rep 2: 213
  3. Kannagi, R. et al. (1983) Stage-specific embryonic antigens (SSEA-3 and -4) are epitopes of a unique globo-series ganglioside EMBO J. 2(12): 2355-2361
  4. Shamblott, M. J. et al. (1999) Proc. Natl. Acad. Sci. U.S.A. 95: 13726-13731
  5. Bhartiya, D. et al. (2012) Very small embryonic-like stem cells with maximum regenerative potential get discarded during cord blood banking and bone marrow processing for autologous stem cell therapy. Stem Cells Dev. 21: 1-6
  6. D'Ippolito, G. et al. (2006) Sustained stromal stem cell self-renewal and osteoblastic differentiation during aging. Rejuvenation Res. 9: 10-19

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