The Anti-MSCA-1 (W8B2) MicroBead Kit, based on the antibody clone W8B2, was developed for the positive selection of mesenchymal stromal cells (MSCs) with a high proliferative capacity, derived from bone marrow aspirates.

Data and images for Anti-MSCA-1 (W8B2) MicroBead Kit, human

Figures

Figure 1

MSCA-1 (W8B2)
+
cells were isolated from human bone marrow mononuclear cells (BM-MNCs) using the Anti-MSCA-1 MicroBead Kit, two MS Columns, and a MiniMACS™ Separator. Cells were stained with Anti-MSCA-1 (W8B2)-APC and CD45-FITC.
Unseparated fraction
MSCA-1
cells
View details

Figure 1

MSCA-1 (W8B2)
+
cells were isolated from human bone marrow mononuclear cells (BM-MNCs) using the Anti-MSCA-1 MicroBead Kit, two MS Columns, and a MiniMACS™ Separator. Cells were stained with Anti-MSCA-1 (W8B2)-APC and CD45-FITC.
View details

Figure 1

MSCA-1 (W8B2)
+
cells were isolated from human bone marrow mononuclear cells (BM-MNCs) using the Anti-MSCA-1 MicroBead Kit, two MS Columns, and a MiniMACS™ Separator. Cells were stained with Anti-MSCA-1 (W8B2)-APC and CD45-FITC.
MSCA-1
+
cells
View details

Figure 1

MSCA-1 (W8B2)
+
cells were isolated from human bone marrow mononuclear cells (BM-MNCs) using the Anti-MSCA-1 MicroBead Kit, two MS Columns, and a MiniMACS™ Separator. Cells were stained with Anti-MSCA-1 (W8B2)-APC and CD45-FITC.

Specifications for Anti-MSCA-1 (W8B2) MicroBead Kit, human

Overview

The Anti-MSCA-1 (W8B2) MicroBead Kit, based on the antibody clone W8B2, was developed for the positive selection of mesenchymal stromal cells (MSCs) with a high proliferative capacity, derived from bone marrow aspirates.

Detailed product information

Background information

The Anti-MSCA-1 (W8B2) MicroBeads recognize the mesenchymal stromal cell antigen-1 (MSCA-1), which is identical to tissue non-specific alkaline phosphatase
1
.
MSCA-1 was shown to be restricted to MSCs in the CD271
bright
population in human bone marrow
2
. These CD271
bright
CD45
dim
MSCs have a much higher clonogenic capacity compared to the CD271
+
CD45
+
fraction in bone marrow. Therefore, MSCA-1 is a highly specific marker to isolate MSCs with a high proliferative potential from bone marrow. MSCA-1 expression was not found on placenta-derived MSCs after culture
3
.
Antibodies against MSCA-1 show broad interspecies cross-reactivity, such as dog, pig, sheep, goat and monkey
4
.

Columns

MS, LS, XS, or autoMACS
®
Columns.

Resources for Anti-MSCA-1 (W8B2) MicroBead Kit, human

Documents and Protocols

References for Anti-MSCA-1 (W8B2) MicroBead Kit, human

Publications

  1. Sobiesiak, M. et al. (2010) The mesenchymal stem cell antigen MSCA-1 is identical to tissue non-specific alkaline phosphatase. Stem Cells Dev. 19: 669-677
  2. Bühring, H. J. et al. (2007) Novel markers for the prospective isolation of human MSC. Ann. N. Y. Acad. Sci. 1106: 262-271
  3. Battula, V. L. et al. (2007) Human placenta and bone marrow derived MSC cultured in serum-free, b-FGF-containing medium express cell surface frizzled-9 and SSEA-4 and give rise to multilineage differentiation. Differentiation 75: 279-291
  4. Rozemuller, H. et al. (2010) Prospective isolation of mesenchymal stem cells from multiple mammalian species using cross-reacting anti-human monoclonal antibodies. Stem Cells Dev. 19: 1911-1921

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