Anti-IgG MicroBeads were developed for the positive selection or depletion of surface IgG-expressing B cell populations from PBMCs or single-cell suspensions from lymphoid tissues.

Data and images for Anti-IgG MicroBeads, human

Figures

Figure 1

IgG
+
memory B cells were separated from PBMCs. CD19
+
B cells were isolated using the CD19 MultiSort Kit. Fc receptor-bound IgG was removed by washing with sodium acetate buffer. Memory B cells were isolated by using Anti-IgG MicroBeads, an MS Column, and a MiniMACS™ Separator.
Pre-selected CD19
+
cells
Isolated surface IgG
+
memory B cells
View details

Figure 1

IgG
+
memory B cells were separated from PBMCs. CD19
+
B cells were isolated using the CD19 MultiSort Kit. Fc receptor-bound IgG was removed by washing with sodium acetate buffer. Memory B cells were isolated by using Anti-IgG MicroBeads, an MS Column, and a MiniMACS™ Separator.
View details

Figure 1

IgG
+
memory B cells were separated from PBMCs. CD19
+
B cells were isolated using the CD19 MultiSort Kit. Fc receptor-bound IgG was removed by washing with sodium acetate buffer. Memory B cells were isolated by using Anti-IgG MicroBeads, an MS Column, and a MiniMACS™ Separator.

Specifications for Anti-IgG MicroBeads, human

Overview

Anti-IgG MicroBeads were developed for the positive selection or depletion of surface IgG-expressing B cell populations from PBMCs or single-cell suspensions from lymphoid tissues.

Detailed product information

Applications

Enrichment or depletion of memory B cells that express surface IgG can be achieved by sequential cell sorting using Anti-IgG MicroBeads. After the enrichment of B cells with the CD19 MultiSort Kit, IgG-expressing memory B cells are specifically labeled and isolated with Anti-IgG MicroBeads.

Columns

For positive selection: MS, LS, XS, autoMACS® Columns, or Multi-24 Column Block. For depletion: LD, CS, D, autoMACS Columns, or Multi-24 Column Block.

References for Anti-IgG MicroBeads, human

Publications

  1. Terada, T. et al. (2001) Fate of the mutated IgG2 heavy chain: lack of expression of mutated membrane-bound IgG2 on the B cell surface in selective IgG2 deficiency. Int. Immunol. 13: 249-256

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Anti-IgG MicroBeads, human

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