Clone:
REA655
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC
Alternative names:
IKZF3, Zfpn1a3, Znfn1a3, 5830411O07Rik

Extended validation for Aiolos Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA655
8B2++
S48-791++
Cells were incubated with an excess of purified unconjugated Aiolos (REA655) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for Aiolos. Splenocytes from C57BL/6 mice were stained with a suitable counterstaining and then fixed and permeabilized using the FoxP3 Staining Buffer Set. Cells were then stained with Aiolos antibodies. As a control, Aiolos antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Aiolos. Splenocytes from C57BL/6 mice were stained with a suitable counterstaining and then fixed and permeabilized using the FoxP3 Staining Buffer Set. Cells were then stained with Aiolos antibodies. As a control, Aiolos antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Aiolos. Splenocytes from C57BL/6 mice were stained with a suitable counterstaining and then fixed and permeabilized using the FoxP3 Staining Buffer Set. Cells were then stained with Aiolos antibodies. As a control, Aiolos antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Aiolos. Splenocytes from C57BL/6 mice were stained with a suitable counterstaining and then fixed and permeabilized using the FoxP3 Staining Buffer Set. Cells were then stained with Aiolos antibodies. As a control, Aiolos antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for Aiolos. Splenocytes from C57BL/6 mice were stained with a suitable counterstaining and then fixed and permeabilized using the FoxP3 Staining Buffer Set. Cells were then stained with Aiolos antibodies. As a control, Aiolos antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Specifications for Aiolos Antibody, anti-mouse, REAfinity™

Overview

Clone REA655 recognizes the mouse Aiolos antigen, a member of the Ikaros family of zinc finger transcription factors, also known as IKZF3. The expression is restricted to lymphoid tissues. Aiolos is highly expressed in spleen and mature B cells and at lower levels in thymus and bone marrow. It was first detected in more committed lymphoid progenitors and strongly up-regulated as these differentiate into pre–T and pre–B cell precursors. It is suggested, that the increase of Ikaros and Aiolos in differentiating lymphocytes is essential for normal progression to a mature and immunocompetent state. Aiolos interacts with Ikaros to regulate lymphocyte differentiation.
Additional information: Clone REA655 displays negligible binding to Fc receptors.

Alternative names

IKZF3, Zfpn1a3, Znfn1a3, 5830411O07Rik

Detailed product information

Technical specifications

CloneREA655
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenAiolos
Alternative names of antigenIKZF3, Zfpn1a3, Znfn1a3, 5830411O07Rik
Molecular mass of antigen [kDa]58
Distribution of antigenB cells, thymocytes, bone marrow, spleen
Entrez Gene ID22780
RRIDAB_2651202, AB_2651203, AB_2651204, AB_2651201

References for Aiolos Antibody, anti-mouse, REAfinity™

Publications

  1. Wang, J. H. et al. (1998) Aiolos regulates B cell activation and maturation to effector state. Immunity 9(4): 543-553
  2. Morgan, B. et al. (1997) Aiolos, a lymphoid restricted transcription factor that interacts with Ikaros to regulate lymphocyte differentiation. EMBO J. 16(8): 2004-2013
  3. Huttlin, E. L. et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143(7): 1174-1189

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