Clone:
105HB29
Type of antibody:
Primary antibodies
Isotype:
mouse IgMκ
Applications:
FC

Specifications for A2B5 Antibody, anti-human/mouse/rat

Overview

Clone 105HB29 recognizes the c-series ganglioside–specific antigen A2B5. The Anti-A2B5 antibody binds to glial progenitors from embryonic to adult human, murine, and rat tissue.
A2B5 is predominantly expressed in embryonic and neonatal neural tissue. In the adult mammalian brain, A2B5 expression is restricted mainly to areas which retain neurogenic potential such as the subventricular zone (SVZ). Thus, A2B5 is considered as a marker for immature glial-committed precursors which are permanently generated in the SVZ. Glial precursor cells are defined as cells that give rise to glial cell types, such as astrocytes and oligodendrocytes.
Ganglioside GT3 and its O-acetylated derivative are the principal A2B5-reactive gangliosides, and both antigens are down-regulated as the cells differentiate into mature oligodendrocytes. Therefore, A2B5 serves as a marker to monitor the maturation of oligodendrocyte progenitors in oligodendrocyte cultures.

Detailed product information

Technical specifications

Clone105HB29
Clonalitymonoclonal
Isotypemouse IgMκ
Isotype controlIsotype Control Antibody, mouse IgM
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman, mouse, rat
AntigenA2B5
RRIDAB_2857622, AB_2660801, AB_10828551, AB_1036062, AB_2857650

Resources for A2B5 Antibody, anti-human/mouse/rat

References for A2B5 Antibody, anti-human/mouse/rat

Publications

  1. Ruffini, F. et al. (2004) Distinctive properties of human adult brain-derived myelin progenitor cells. Am. J. Pathol. 165: 2167-2175
  2. Dietrich, J. et al. (2002)
    Characterization of A2B5
    +
    glial precursor cells from cryopreserved human fetal brain progenitor cells.
    Glia 40: 65-77
  3. Dubois, C. et al. (1990) Monoclonal antibody A2B5, which detects cell surface antigens, binds to ganglioside GT3 (II3 (NeuAc)3LacCer) and to its 9-O-acetylated derivative. J. Biol. Chem. 265: 2797-2803
  4. Larsen, P. H. et al. (2003) Matrix metalloproteinase-9 facilitates remyelination in part by processing the inhibitory NG2 proteoglycan. J. Neurosci. 23: 11127-11135
  5. Larsen, P. H. and Yong, V. W. (2004) The expression of matrix metalloproteinase-12 by oligodendrocytes regulates their maturation and morphological differentiation. J. Neurosci. 24: 7597-7603
  6. Larsen, P. H. et al. (2006) Myelin formation during development of the CNS is delayed in matrix metalloproteinase-9 and -12 null mice. J. Neurosci. 26: 2207-2214
  7. Najm, F. J. et al. (2013) Transcription factor-mediated reprogramming of fibroblasts to expandable, myelinogenic oligodendrocyte progenitor cells. Nat. Biotechnol. 31(5): 426-433
  8. Windrem, M. et al. (2004) Corrigendum: Fetal and adult human oligodendrocyte progenitor cell isolates myelinate the congenitally dysmyelinated brain. Nat Med 10: 209
  9. Windrem, M. (2004) Fetal and adult human oligodendrocyte progenitor cell isolates myelinate the congenitally dysmyelinated brain. Nat Med 10: 93-97
  10. Albert, M. et al. (2013) The histone demethylase Jarid1b ensures faithful mouse development by protecting developmental genes from aberrant H3K4me3. PLoS Genet. 9(4): e1003461

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