Comprehensive solutions for your Treg human and mouse research

 

  • Fast and gentle isolation and flow sorting of Treg cells and distinct  Treg subsets
  • Optimized culture, activation, and expansion of Treg cells
  • Phenotyping and functional analysis of Treg cells

Application protocols
 

We offer comprehensive solutions for each step of your Treg research studies. Our workflows are synergistic and fully translational to clinical Treg applications.

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Comprehensive solutions for your Treg mouse and human research

Regulatory T cells (Tregs) are an important T cell subpopulation that maintains immunological homeostasis. Today, Treg-based therapy shows an enormous potential for treatment of autoimmune and auto-inflammatory disorders, acute and chronic infection, allergy, metabolic inflammation, transplantation, and cancer. Therefore, it is crucial to provide reliable and trustworthy research materials alongside an easily translatable workflow into the clinic. This webinar explores our innovative tools to support Treg research at every step.

Research Treg workflow steps

Sample preparation for Tregs

Tregs from mouse tissue

Tregs represent 1–4% of all lymphocytes in secondary lymphoid organs (such as spleen and lymph node) of wild type mice. Spleen and lymph nodes must be dissociated into a single-cell suspension for many downstream applications. Dissociation can be accomplished with specific Tissue Dissociation Kits (e.g. Spleen Dissociation Kit, mouse) manually or in a fast and convenient way by using the automatic gentleMACS™ Dissociator.

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Analysis of CD4+FoxP3+Treg cells in cell suspensions of lamina propria from mouse colons by flow cytometry using Flowlogic™ software.

 

Isolation and cell sorting of Tregs

Isolation

We offer a broad range of products for the isolation of Treg cells according to your starting material and species; human, mouse, and non-human primate. MACS® MicroBeads and Isolation Kits are the best products for isolation of highly pure and viable distinct Treg subsets.

 

MACS® MicroBead Technology is the most trusted and proven cell isolation technology.

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Isolation of human CD4+CD25+CD127dim/-Regulatory T Cell using MACS® Technology

  • Minimal labeling and smallest bead size – preserved characteristics and no cell activation.
  • No cell stress – highest viability and no effects on cell functionality.
  • Free epitopes and no bead aggregates – cells can be directly used in downstream applications.
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Isolation of mouse Treg cells using the CD4+CD25+ Regulatory T Cell Isolation Kit, mouse

Our MACSxpress® Treg Isolation Kit, human has been developed for the isolation of CD4+CD25+ Treg cells from up to 30 mL of freshly drawn anti-coagulated whole blood. Isolate pure Treg cells in only 30 minutes, without the requirement of density gradient centrifugation. 

Sorting of Tregs with MACSQuant® Tyto® Cell Sorter
 

Tregs are fragile and sensitive to mechanical stress. Therefore, gentle isolation is a critical step for accurate downstream (functional and phenotypical) analyses and applications.

The MACSQuant® Tyto® Cell Sorter is revolutionizing cell sorting. Our patented microchip-based technology opens up new possibilities in basic research and medical applications with high-speed multiparameter cell sorting in the safety of a fully enclosed and sterile cartridge system, the MACSQuant Tyto Cartridge.

The MACSQuant Tyto Cell Sorter gives you the opportunity to sort different subtype of Tregs not only for your research purposes but also for cell and gene therapy application. 
 

Treg cell sorting with MACSQuant® Tyto® Cell Sorter.

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Human Treg sorting by MACSQuant® Tyto® CellSorter. 

  • Gentle to cells: Sort and even resort cells under low pressure without compromising cell viability or functionality.
  • Fast and easy handling: No drop delay or laser alignment needed.
  • Full sterility: Contamination-free within the disposable, fully closed MACSQuant Tyto Cartridge.

REAfinity™ Recombinant Antibodies provide complete multiparameter labeling for your Tregs research Flow cytometry and cell sorting analysis. However, REAlease® Fluorochrome Technology not only supports you with complete multiparameter labeling, but provides unlabeled Tregs after the cell sorting.

Culturing, activation and expansion of Tregs 

Activation, expansion, and stimulation of Treg cells can be very challenging. The combination of TexMACS™ Medium, MACS® Cytokines, and Treg expansion kits (human and mouse) are perfectly suited for in vitro culture and expansion of Treg cells.

TexMACS™ Medium is an optimized serum-free cell culture medium developed for the cultivation and expansion of human and mouse T cells and regulatory T cells.
 

Optimal expansion of your isolated Treg cells using Treg Expansion Kit

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Expansion of Treg cells using the Treg Expansion Kit, human. 

  • Based on cell-sized MACSiBead™ Particles loaded with CD3 and CD28 antibodies
  • Optimized to perfection and a careful balance of the stimuli enables reliable Tregs expansion
  • Maintenance of Tregs phenotype and FoxP3 expression in vitro.

Treg analysis 

Treg suppression assay

The Treg Suppression Inspector has been developed for the in vitro functional characterization of human CD4+CD25+regulatory T cells.

Treg in vitro suppression assay

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Treg suppression assay using the Treg Suppression Inspector, human.

  • An optimized T cell stimulation reagent for a Treg suppression assay
  • Based on Anti-Biotin MACSiBead™ Particles
  • Analysis of Tregs suppression activity by reduction of responder T cells proliferation

Flow cytometry analysis

Treg Detection Kits (mouse and human)

The Treg Detection Kits are optimized antibody kits with pre-titrated antibodies that ensure a reliable flow cytometric analysis of Treg cells. Kits are ready-to-use cocktail, which conveniently reduces pipetting steps.

REAfinity™ Recombinant Antibodies

REAfinity™ Antibodies are recombinant antibodies that provide superior lot-to-lot consistency and purity compared to mouse or rat hybridoma-derived, monoclonal antibodies.

Treg characterization with REAfinity™ Antibodies

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REAfinity™ Recombinant Antibody model

  • Greater reproducibility: High purity and lot-to-lot consistency with less background
  • Easy handling: Eliminates tedious and costly Fc receptor blocking steps
  • One universal isotype: Convenient and cost-saving
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Gating strategy for identification of different Treg cells subsets

 

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Scientific poster
Vio® Dyes meet REAfinity™ Antibodies – antibody-fluorochrome conjugates for high-resolution multiparameter flow cytometry analysis

Cesar Evaristo, Marco Vahldieck, Susanne Krauthaeuser, Nicole Jansen, Niklas Wilske, Anne Richter, and Christian Dose, Miltenyi Biotec B.V. & Co. KG Bergisch Gladbach, Germany
 

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