Optimize ex vivo T cell activation and expansion in translational research, while preserving functionality and viability. After gentle T cell isolation with our MACS® Technology solutions, activation can be either performed polyclonally or antigen specific. Efficient and reliable proliferation ensures viable and functional T cells for any downstream application. Our flow cytometry solutions enable highly accurate multiparameter T cell analysis.
Reliability now comes with better performance and faster protocols. Our improved MicroBeads and T Cell Isolation Kits allow for high-quality separation of human and mouse T cells within a few minutes.
StraightFrom® MicroBeads allow magnetic cell separation directly from human blood products, such as whole blood, buffy coat, leukoreduction system chambers (LRSCs) and leukopaks®. CD4+ and CD8+ T cell subsets can be purified using the autoMACS® Pro Separator, the MultiMACS™ Cell24 Separator Plus, or manual separators.
REAlease® Technology allows for magnetic cell isolation with subsequent removal of any beads and labels from the cell population of interest. Clever utilization of recombinant antibody fragments allows for cell surface labeling in just a few steps.
The technology relies on recombinantly engineered antibody fragments for cell surface labeling and thus achieves highly reproducible results. The REAlease Biotin Complex binds to the target cells. Labeling of the REAlease Biotin Complex with Anti-Biotin MicroBeads allows for magnetic isolation of these cells. Following cell separation, both MicroBeads and REAlease Biotin Complex are gently removed, leaving the cells bead-and label-free. Enriched cells are then suitable for magnetic re-labeling and any application where label-free cells are essential.
Philipp Steinbrueck, Zhong Yu, Ursula Bissa, Jennifer Brieden, and Christian Dose
TexMACS™ Medium is an optimized, serum-free cell culture medium developed for reliable T cell and Treg cell culture results. Chemically defined and without animal-derived components, it allows for the highest reproducibility in human and mouse cell culture applications.
We offer a variety of polyclonal and antigen-specific T cell stimulation tools to choose from.
Non-toxic alternative to the superantigen SEB for the stimulation of human T cells.
As the final step in many workflows, flow analysis plays an important part in various T cell applications. The following flow reagents make sure that flow cytometry data is reliable and consistent:
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