MBTP 23: Immunophenotyping of cortical neurons from mouse brain using flow cytometry

Miltenyi Biotec-tested panel 23 (MBTP 23)

This application protocol describes the quantitative analysis of mouse cortical neurons upon isolation and culture using flow cytometry. Mouse brains are easily dissociated using the gentleMACS™ Dissociators to quickly obtain viable single cell suspensions ready for flow cytometric analysis.

Protocol

Gating strategy showing the analysis of mouse cortical neurons. Cortical neurons from mouse brains were stained using the previously described panel for quantitative analysis. Samples were gated on live cells using SSC-A/FSC-A gating (A). Single cells were gated using FSC-H/FSC-A gating (B). Propidium iodide staining was used for exclusion of dead cells (C). Neuron cell purity was measured by analyzing other cell type contaminants. O4+ cells (D), ACSA-2+ cells (E), and CD45+ CD11b+ cells (F) were defined as oligodendrocytes, astrocytes, and microglia, respectively. 

Materials

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