| Description |
Prominin-1 is a 115–120 kDa, 5-transmembrane glycoprotein with close structural similarity to its human orthologue, CD1331. Prominin-1 expression has been demonstrated on cells of different embryonic and adult tissues, including ES-derived committed and early progenitor cells2, the developing3 and postnatal4 central nervous system, kidney tubules3, and cells of the hematopoietic system3. In the developing nervous system prominin-1 is predominantly expressed on neuroepithelial cells and other epithelia. In the postnatal brain, prominin-1 expression has been found in areas of neurogenesis, such as the subventricular zone (SVZ). Prominin-1 is preferentially localized to plasma membrane protrusions of the apical membrane.5
|
| Applications |
| Prominin-1+ cells selected by MACS® Technology can be used, e.g. for studying differentiation potential. Prominin-1+/Lineage- cells isolated from postnatal cerebellum were reported to be capable of differentiating into neurons, astrocytes and oligodendrocytes in vitro and in vivo.4 |
| |
| Figure 1 |
| Enrichment of Prominin-1+ cells from mouse cerebellum (postnatal day 1) using Anti-Prominin-1 MicroBeads, a MiniMACS Separator and MS Columns. |
| Mouse cerebellar cells before separation |
 |
| Mouse cerebellar cells after depletion of Prominin-1+ cells |
 |
| Enriched Prominin-1+ cells |
 |
|
|
