Anti-mPDCA-1 - functional grade抗体 (マウス)

Overview

Anti-mPDCA-1 pure, mouse – functional grade は、マウス実験モデルにおける PDC の機能的役割の研究のために、PDC の in vivo でのディプリーションができるように開発されたました。この抗体は、抗ウイルス反応にPDCsが重大な役割を担っていると示唆されている疾患モデルマウスの、マウスCMV²、皮膚HSV³、膣HSV-2⁴などにおける、PDCsのディプリーションに使用されています。
さらに、リステリア菌感染後のconventional DCsとPDCs間の相互作用が、CpGによって引き起こされる免疫反応の活性化に重要であると明らかになりました。

Details

Background information
クローンJF05-1C2.4.1は、Mouse plasmacytoid dendritic cell antigen-1 (mPDCA-1) を認識します。mPDCA-1はマウスplasmacytoid dendritic cells (PDCs) に特異的に発現しています。PDCs は、CD11c⁺樹状細胞のサブセットで、すべてのリンパ組織、末梢血、そして一部の非リンパ組織において低頻度で見られます。マウス脾臓、骨髄、リンパ節において、mPDCA-1 は CD11c^low, CD45R (B220)⁺, Ly-6C^high, MHC Class II^low, CD8a^low/-, CD80^low/-, CD86^-, CD40^-, CD11b^-, CD90^-, CD49b (DX5)^-, CD3^-, CD19^-、すなわちマウス PDCs の表現型をもつ細胞にのみ特異的に発現しています。脾臓細胞の蛍光多重染色により、すべての CD11c⁺ CD45R (B220)⁺ Ly-6C⁺ PDCs は mPDCA-1⁺ で、mPDCA-1 の発現は PDCs に特異的であることが明らかとなりました。

Clone	Isotype
JF05-1C2.4.1	Rat IgG2b

Figure 1

Mouse PDCs were specifically depleted in BALB/c mice by i.p. injection of 500 µg Anti-mPDCA-1 pure – functional grade. The frequency of mPDCA-1⁺ PDCs was analyzed in spleen 24 h after injection of the antibody or diluent only (control mice) by staining with Anti-mPDCA-1. To exclude blocking of Anti-mPDCA-1 staining by the in vivo applied Anti-mPDCA-1 antibody, cells were additionally stained with Anti-Ly-6C and CD45R (B220). Note that the frequency of PDCs decreases in mice which received the Anti-mPDCA-1 antibody (A) to about one fifth compared to control mice (B), independent of whether it is analyzed based on Anti-mPDCA-1 staining or on Anti-Ly-6C and CD45R (B220) staining. Cell debris and dead cells were excluded from the analysis based on scatter signals and PI fluorescence.

A) Anti-mPDCA-1 antibody

Mouse PDCs were specifically depleted in BALB/c mice by i.p. injection of 500 µg Anti-mPDCA-1 pure – functional grade. The frequency of mPDCA-1<sup>+</sup> PDCs was analyzed in spleen 24 h after injection of the antibody or diluent only (control mice) by staining with Anti-mPDCA-1. To exclude blocking of Anti-mPDCA-1 staining by the <i>in vivo</i> applied Anti-mPDCA-1 antibody, cells were additionally stained with Anti-Ly-6C and CD45R (B220). Note that the frequency of PDCs decreases in mice which received the Anti-mPDCA-1 antibody (A) to about one fifth compared to control mice (B), independent of whether it is analyzed based on Anti-mPDCA-1 staining or on Anti-Ly-6C and CD45R (B220) staining. Cell debris and dead cells were excluded from the analysis based on scatter signals and PI fluorescence.

B) Control