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Miltenyi Biotec
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Miltenyi Biotec
Miltenyi Biotec
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Miltenyi Biotec
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gentleMACS™ Protocols for sample preparation
Preparation of single-cell suspensions from mouse spleen without enzymatic treatmentPDF, 71 KB
Preparation of single-cell suspensions from mouse liverPDF, 91 KB
Preparation of single-cell suspensions from mouse heartPDF, 95 KB
Preparation of single-cell suspensions from implanted mouse tumorsPDF, 86 KB
Homogenization of tissue for total RNA isolationPDF, 71 KB
Homogenization of tissue for mRNA isolationPDF, 74 KB
Homogenization of tissue for protein extractionPDF, 68 KB
Preparation of mitochondria from mouse tissue with enzymatic treatmentPDF, 129 KB
Further protocols for sample preparation
Isolation of mononuclear cells from human peripheral blood by density gradient centrifugationPDF, 89 KB
Isolation of mononuclear cells from human cord blood by density gradient centrifugationPDF, 74 KB
Isolation of mononuclear cells from human bone marrow aspirates by density gradient centrifugationPDF, 76 KB
Isolation of granulocytes from human peripheral blood by density gradient centrifugationPDF, 83 KB
Preparation of cells from lymphoid tissuePDF, 67 KB
Preparation of single-cell suspensions from mouse bone marrowPDF, 58 KB
Preparation of the stromal vascular fraction (SVF) from human lipoaspiratePDF, 117 KB
Isolation of human dermal microvascular endothelial cells (HDMECs) from foreskin tissuePDF, 435 KB
Isolation of human umbilical vein endothelial cells (HUVECs) from umbilical cordPDF, 195 KB
Dissection of retinasPDF, 72 KB
Special protocols for cell separation
Isolation of CD34+HLA-DR- cellsPDF, 72 KB
Isolation of CD34+CD38- cellsPDF, 75 KB
Isolation of CD271 (LNGFR)+ MSCs/ADSCs from human lipoaspiratePDF, 302 KB
Isolation of CD4+ T helper cells from human PBMCsPDF, 63 KB
Isolation of CD4+CD279+ T cells from human PBMCsPDF, 347 KB
Isolation of CD4+CD294 (CRTH2)+ Th2 cells from human PBMCsPDF, 265 KB
Isolation of CD4+CD62L+ T cells from human PBMCsPDF, 303 KB
Isolation of CD8+CD62L+ T cells from human PBMCsPDF, 345 KB
Isolation of CD8+CD279+ T cells from human PBMCsPDF, 351 KB
Enrichment of CD134 (OX40)+ cellsPDF, 265 KB
Isolation of CD10+ B cells from human bone marrow or lysed bloodPDF, 417 KB
Isolation of CD138 plasma cells from human PBMCsPDF, 300 KB
Isolation of CD105+Sca-1+ LTR-HSCs from mouse bone marrowPDF, 192 KB
Isolation of iNKT cells from mouse spleen using CD1d tetramersPDF, 252 KB
Isolation of naive CD4+ T cells from non-human primate PBMCsPDF, 214 KB
Isolation of CD4+CD25+CD127dim/– regulatory T cells from non-human primate PBMCsPDF, 527 KB
Isolation of rat B lymphocytesPDF, 99 KB
Schwann cell isolation from rat peripheral nerve tissue biopsiesPDF, 159 KB
Positive Selection of Antibody Expressing Hybridoma CellsPDF, 77 KB
Special protocols for Cytokine Secretion Assays
Detection of cytokine-secreting cellsPDF, 165 KB
Detection and enrichment of cytokine-secreting cellsPDF, 202 KB
Two-color Cytokine Secretion AssaysPDF, 98 KB
Combined staining of cytokine-secreting cells with peptide-MHC tetramersPDF, 102 KB
Detection of cytokine-secreting cells from whole bloodPDF, 71 KB
Detection and enrichment of cytokine-secreting cells from whole bloodPDF, 142 KB
Expansion of antigen-specific cytokine-secreting T cellsPDF, 275 KB
Special protocols for cell analysis
Immunohistochemical detection of CD133 in paraffin-embedded sectionsPDF, 658 KB
Special protocols for cell culture
NH ChondroDiff Medium - Differentiation of MSCs into chondrocytes and their detectionPDF, 98 KB
Special protocols for molecular applications
Chromatin immunoprecipitation (ChIP)PDF, 94 KB
cDNA synthesis from whole blood using the μMACS cDNA Kit and subsequent KIR typingPDF, 92 KB
Isolation of mRNA from stablized whole bloodPDF, 111 KB
Isolation of mRNA from yeast cellsPDF, 74 KB
Isolation of specific transcripts/RNAsPDF, 107 KB
Isolation of specific tRNA moleculesPDF, 116 KB
Isolation of RNA-binding proteinsPDF, 128 KB
µMACS™ Streptavidin Kit—tips & hintsPDF, 91 KB
Biotinylation of plasmidsPDF, 47 KB
Subtractive hybridizationPDF, 88 KB
Phage displayPDF, 96 KB
Isolation of infectious HIV-1PDF, 106 KB
Isolation of infectious HIV using stimulated PBMCsPDF, 162 KB
MACSelect Tag Vector cloning tipsPDF, 171 KB
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