Human ES/iPS cell research


This 3.5-day training course focuses on improving pluripotent stem cell generation, culture and characterization. The course covers all critical steps required for pluripotent stem cell research. Encompassing theoretical presentations and practical lab sessions, participants will learn advanced ways of cell preparation, cell selection with magnetic cell separation and basic cell analysis with flow cytometry.

Session overview
Start DateTuesday, March 25, 2014 - 1:00 PM
End DateFriday, March 28, 2014 - 4:00 PM
Max. Participants8
Min. Participants4
TrainerDr. Sebastian Knöbel, Dr. Frank Hardung
Price2.500,00 €
LocationBergisch Gladbach/Cologne, Germany
Order No.160-001-640
Target groupPhD students, postdoctoral fellows, research assistants and technicians interested in ES/iPS cell research and with experience in basic cell culture.

Enrollment deadline: 3 weeks in advance.

Places availableYes
Course lectures and exercises include:
  • Reprogramming techniques for the generation of iPS cell lines
  • Characterization of pluripotent stem cells with different methods
  • Cultivation strategies of pluripotent stem cells
  • Pluripotent stem cell differentiation
  • Basics of magnetic cell separation for pluripotent stem cell cultures
  • Basics of flow cytometry
  • Discussion and trouble-shooting with experienced users from R&D
 Practical laboratory parts include:
  • Setup and maintenance of iPS cell cultures
  • Transfection procedure for mRNA-based reprogramming
  • Hands-on introduction to flow cytometry
  • iPS cell characterization with different methods, including flow cytometry
  • Magnetic separation of differentiated from undifferentiated iPS cells
  • Removal of feeder cells with magnetic separation
  • Improved dissociation of murine embryoid bodies

Note: All practical parts are performed using human and murine pluripotent stem cell lines.

Learning objectives
  • Get to know new technologies for ES/iPS cell research
  • Be able to setup and maintain pluripotent stem cell cultures
  • Learn how to improve results with magnetic cell separation
  • Understand principles of flow cytometry in the context of ES/iPS cell research
  • Apply an easy and safe method for cell reprogramming
  • Acquire a new approach to embryoid bodies dissociation

Basic cell culture experience is required.

Training includes
  • Training materials
  • Certificate of successful course completion
  • Hotel accommodation (four nights)
  • Lunches, two hosted dinners, and a special event
  • Transportation to/from designated hotel to/from MACS Academy.

Enrollment deadline: 3 weeks in advance.

Location specify
MACS Academy at Miltenyi Biotec Headquarters
Friedrich-Ebert-Strasse 68
51429 Bergisch Gladbach, Germany
Phone: +49 2204 8306-0

Contact email:


Please check for further courses scheduled at MACS Academy.

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Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 877 591 1060
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