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Learn at your own pace. Our online tutorials cover a wide variety of topics and are available to our customers anytime, anywhere.

With our online tutorials, you can start, pause, and stop your courses whenever you want – as well as access only the specific content from each tutorial that is relevant to you. Recordings and handouts from our live webinars are also available. Online tutorials are a convenient way for all levels of users to stay up to date in their field.

Take your online tutorial today, or feel free to contact us directly with any questions or requests: MACS_Academy@miltenyibiotec.de

Start smart with your sample preparation – isolation of immune cells from mouse tissue

Perfectly prepared single-cell suspensions with high cell viability and a minimum of unwanted material generate excellent results in cellular analysis, and cell culture. Miltenyi Biotec has developed a pioneering tissue dissociation technology with the gentleMACS™ Dissociators, Tissue Dissociation Kits and their unique gentleMACS Tubes. In this webinar (November 2017), we will introduce you to the application of the gentleMACS technology in immunology research, focusing on the preparation of immune cells from mouse lung, intestine and central nervous system (CNS). Request the presentation (pdf) or watch the recording .

Challenges in NK cell immunotherapy: where do we stand?
This webinar will review the current status of NK cell-based immunotherapy. Martha Elia Luevano Salinas, product manager NK cells, will discuss different strategies used to generate or isolate NK cells for immunotherapy and current approaches boosting NK cell cytotoxicity. Please request the presentation or watch the webinar recording (Oct 2016).

Dendritic cells (DC) therapy reloaded
This episode of the translational webinar series reviews the relevance of DC-based therapy and shows innovative tools to develop therapeutic approaches based on primary DCs. Emiliano Melandri, PhD, covers state-of-the-art tools for vaccine development up to complete solutions for DC subset research. Please request the presentation or watch the webinar recording (Oct 2016).

Identification and kinetics of circulating follicular human T helper subpopulations
Watch in this webinar how the MACSQuant® Instrument, with its integrated pre-enrichment column, allowed Dr. Elke Bergmann to analyse in detail rare antigen specific CXCR5+ T cells after vaccine administration, gaining novel insights into the fight against Ebola. Please request the presentation or watch the webinar recording (Sep 2016).

Spotlights on T cells: new developments for translational and basic T cell research
In this seminar, you will gain insights into how T cells can be studied using techniques for isolation of T cells or their subsets out of cell samples, in vitro differentiation, stimulation, and culture methods, as well as highly sensitive flow cytometry assays. Please request the presentation or watch the webinar recording (July 2016).

Mo-DC: the next generation
This webinar, as part of the “translational research” series, aims to give you an overview of DC-based clinical research. We will guide you through the challenges and solutions of Mo-DC manufacturing for clinical trials and help you bring your DC translational research to the next level. Please request the presentation or watch the webinar recording (June 2016).

Dendritic cells: all you need is blood
Dendritic cells (DCs) are a type of leukocytes that play a key role in our immune system. They link the innate and adaptive immunity and are the most potent antigen-presenting cell type. This webinar (September 2015) explains how to investigate peripheral blood derived DC and give an overview of DC subsets in human peripheral blood. Watch the webinar recording or request the presentation (pdf).

Immunophenotyping – get the most out of your flow cytometry analysis
Do you want to learn how to set up a multicolor panel and analyze cell subsets from PBMC or whole blood samples? Immunophenotyping by flow cytometry provides a basic overview of blood sample compositions with respect to the major cell types in seconds. To get the most out of your analysis, however, it is essential to properly design the panel and the controls. Here it is shown how to perform multicolor analysis of leukocyte subsets. Request the presentation or watch the recording of this webinar (May 2013).

Tailor-made solutions for NK cell isolation and analysis
To demonstrate tailor-made research tools for NK cells, Dr. Claudia Loske, Product Manager Immunology describes in this webinar workflows from recent peer-reviewed publications and addresses applications such as cell separation, cell phenotyping, and expression analysis. Request the presentation or watch the recording of this webinar (Nov 2011).

Generation of highly enriched hiPSC-CMs for high-throughput cardiotoxicity screens
Human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs) are emerging as an ideal in vitro platform for pre-clinical cardiotoxicity screening of new chemical entities. In this webinar, Todd J. Herron, PhD, Director of the Cardiovascular Regeneration Core Laboratory, University of Michigan, shows how they utilized a non-genetic approach to generate highly enriched hiPSC-CMs using magnetic cell sorting to deplete non-cardiomyocytes from the cell population. Watch the recording or request the presentation (pdf) of this webinar (June 2017).

Clinical scale cultivation of MSCs – Tools to support an GMP-compliant workflow
The demand for xeno-free and GMP-compliant production of clinical-scale mesenchymal stem cells (MSCs) as well as standardized quality control measures for cellular products is rising. Kathrin Godthardt, Senior Scientist, R&D Department, Miltenyi Biotec, shows xeno-free MSC expansion solutions and workflows for qualifying the quality and phenotype of culture expanded MSCs. Furthermore, the CliniMACS Prodigy will be introduced as an automated closed system for GMP conform isolation and expansion of stem cells. Watch the recording or request the presentation (pdf) of this webinar (November 2016).

HSC-CFU assays
Want to get started with HSC-CFU assays? This free, interactive online training with an interactive section for practicing colony identification online, will help you to understand the basic protocol and to learn how to set up your assays and avoid common pitfalls.
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Novel tools for cardiac cell isolation
Neonatal cardiomyocytes (CMs) as well as cardiomyocytes derived from pluripotent stem cells (PSC) are commonly used to study biochemical, physiological, pharmacological, or morphological characteristics or investigat biochemical pathways. Gregor von Levetzow introduces in this webinar (May 2016) novel tools for cardiovascular research. Watch the webinar recording or request the presentation (pdf) and see how easy primary CMs can be highly enriched from neonatal hearts or differentiated PSC cultures.

Foundations of stem cell maintenance
In this webinar, we’ll guide you through the different opportunities to culture cells and test their quality. Learn more about cell splitting techniques and find out how to sort pluripotent stem cells for continuous, high-level quality. Request the presentation (pdf) or watch the webinar recording (April 2016).

Foundations for stem cell reprogramming
Foundations for stem cell reprogramming is the first in a series of webinars on stem cell research. The 45-minute webinar introduces a highly efficient method for reprogramming into iPS cells and will deepen your stem cell knowledge. Request the presentation (pdf) or watch the webinar recording (August 2015).

Quantitative and qualitative analysis of ES and iPS cell culture by flow cytometry
Stem cells express a number of proteins that are considered to be markers of pluripotency. The differentiation of stem cells or the reprogramming of cells to iPS cells leads to changes in these markers. The – qualitative and quantitative – condition of a stem cell population can therefore be tracked using fluorescently tagged antibodies against these markers and flow cytometry. Sebastian Knöbel, PhD, Senior Project Manager Miltenyi Biotec R&D, shows examples of cell culture monitoring by flow cytometry and explains the critical factors to be considered for a valid analysis. Request the presentation of this webinar (November 2013) or watch the recording.

Integrated solutions for neuroscience research − Adult brain dissociation and neural cell isolation
The isolation of pure, viable and functional adult neural cells provides an important tool for fully understanding the biology of adult neural cells, disease mechanisms e.g. in neurodegenerative diseases and for drug screening. Miltenyi Biotec has developed dedicated workflows to investigate adult rodent neural cells that include brain dissociation, isolation of neurons, astrocytes, microglia, oligodendrocytes, and endothelial cells, cultivation and flow cytometry analysis in only one day. In this webinar (July 2017), you will get to know how innovative technologies are being implemented in our adult brain workflows, and how we can help to advance neuroscience research. Watch the webinar recording or request the presentation (pdf).

Better Methods for Neural Cell Isolation
With this webinar from May 2012, you can learn the latest methods, technologies, and strategies for neural cell isolation. Discover new techniques that are gentle, efficient, sterile, reproducible, and most of all, effective. Watch the webinar recording or request the presentation (pdf) of Dr. Anthony Allen, Global Product Manager Miltenyi Biotec.

Novel technologies for improved neural sample preparation
Neural sample preparation is a critical but often overlooked step of every neuroscience experiment. In this webinar (Feb 2012) we will show an easy, fast, and automated sample preparation procedure. Additionally, experimental data demonstrate how quality of results improve using MACS Cell Separation to remove myelin or isolate specific neural cell populations. Watch the webinar recording or request the presentation (pdf).

Cancer

Advancing tumor research: a revolutionary workflow for isolating pure cell populations from primary tumor tissue
Tumor tissue is infiltrated by non-tumor cells (TILs) in such a way, that the comparison of even two identical tumor types in the same tissue will show very high differences in levels of infiltration. To make these samples comparable and useful for any downstream application, it is crucial to have pure cell populations of your cells of interest. In this webinar (June 2017) we will explain the complete workflow for dissociating human, mouse and xenograft tissue into single cell suspensions and show how to specifically separate tumor cells from non-tumor cells. Request the presentation  or watch the recording.

Isolating tumor cells for improved downstream analysis
When analyzing human tumor tissue, it is essential for any downstream application to clearly differentiate between tumor and non-tumor cells. This can be a difficult task. Olaf Hardt, R&D Project Manager at Miltenyi Biotec, demonstrates in this webinar recording (September 2015) fast and easy solutions to distinctly separate human tumor cells from non-tumor cells. Watch the webinar and facilitate your tumor research, receive standardized protocols and get to know optimized strategies for the isolation of tumor cells. Request the presentation  or watch the recording.

Reliable Analysis of Tumor Subpopulations
Performing research with tumor samples brings special challenges. Samples can be heterogeneous, difficult to dissociate, and complex to analyze. Olaf Hardt, R&D Project Manager at Miltenyi Biotec, demonstrates in this webinar (March 2013) a standardized tumor analysis workflow and shares tips to get the most reliable data from your samples. Request the presentation  or watch the recording to see how gentle and standardized tumor dissociation conserves cell surface epitopes for downstream analysis. Learn how flow cytometry, a powerful yet underrepresented method in tumor analysis, provides fast and reliable results.

Highlight tumor development in small animal models using optimized contrast agents
Discover the efficiency of Viscover™ Imaging Agents, NiraWave™ M for optical imaging and ExiTron™ nanoparticles for computed tomography (CT), that were specially developed for prolonged blood circulation and high contrast images. Request the presentation (pdf) or watch the recording from August 2012 to see data from tumor analysis in small animals. 

Multicolor flow cytometry – Understand the basics to be the expert
The aim of this 45-minute webinar by Guerric Epron, Miltenyi Biotec, is to explain technical basics and how they affect multicolor flow cytometry: Why is a fluorochrome bright or dim? How do you compensate? What is the data-spread effect and can you overcome it? Understand the essential steps for high quality results and interpretation - accurate selection of fluorochromes, consideration of antigen density and setting your flow cytometer properly. Request the presentation or watch the recording.

Recombinant antibodies for improved reproducibility in flow cytometry analysis
Antibodies are key reagents for numerous cell analysis applications. Recently, there were a lot of discussions on irreproducible results due to the inconsistency of antibodies. Recombinantly generated antibodies, such as the REAfinity™ Antibodies, possess highly defined characteristics conditions leading to reproducible results. Request the presentation (pdf) or watch the webinar (March 2017) to understand the common problems associated with antibodies in flow cytometry and the advantages of REAfinity Antibodies.

MACS Quantify software version 2.8
The MACSQuantify™ Software is a powerful tool for acquisition and analysis of flow cytometric data on the MACSQuant® Flow Cytometers. This 45-minute webinar presents the new features of the MACSQuantify Software version 2.8 that will improve your flow cytometric analyses. Watch the webinar recording or request the presentation (August 2016).

Exosome analysis using a multiplex bead-based flow cytometry assay
Stefan Wild, senior scientist R&D at Miltenyi Biotec introduces in this webinar (April 2016) a multiplex bead-based flow cytometry assay for exosome analysis. Watch the webinar and see how easy EVs can be distinguished by surface marker profiling and how selected markers can be used to separate EV populations for further downstream analysis. Watch the webinar recording or request the presentation (pdf).

How to optimize sample preparation for flow cytometry
Careful preparation of cell samples is the first crucial step towards reliable flow cytometric analysis. Suspensions of single, viable cells are a prerequisite for obtaining optimal results. In this technical presentation we will discuss how to prepare samples from diverse specimens like cultured cells, blood, or tissue. Request the presentation (pdf) or watch the webinar recording (Jan 2016).

Analysis of intracellular signalling using flow cytometry
Please request the presentation or watch the recording to learn how to set up a successful phospho-flow analysis. In this 1h webinar we will present already optimised reagents that will allow you an easy and fast analysis of intracellular signalling.

Multicolor Flow Cytometry
Multicolor flow cytometric analysis of cells provides a wealth of information from even small samples. This webinar (July 2015) explains how to optimally apply compensation to multicolor analysis and which controls have to be done. Request the presentation or watch the recording.

Rare cell analysis in flow cytometry
This webinar (October 2014) highlights how to set up rare cell experiments in flow cytometry, including sample labeling and instrument setup. Additional topics include how to overcome common challenges and extend the detection limit of conventional flow analysis using pre-enrichment. Please request the presentation or watch the recording.

Get the colors right: Compensation of spectral overlap in flow cytometry
Multicolor flow cytometric analysis of cells provides a wealth of information from even small samples. However, spectral overlap can cause false positive events. Compensation can be applied to overcome this problem and help generate accurate data. This webinar (February 2014) explains how to optimally apply compensation to multicolor analysis. Using examples of typical fluorochromes, specific compensation issues and their resolution will be addressed. Request the presentation or watch the recording.

MACSQuantify Software - interactive tutorials
MACSQuant® Instruments, our ultra-compact flow cytometers that enable multisample and multiparameter sample analysis, use the MACSQuantify Software. These short tutorials will help you navigate through the MACSQuantify Software and learn how comprehensive and easy flow analysis can be. Current topics are absolute cell counting and automated compensation.
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Save time and effort in your flow cytometry experiments
Shorten assay set up and problem solving time with reagent kits and cocktails designed with the busy scientist in mind. Take advantage of flow cytometry reagents, kits and assays that are fast and easy to implement in your every day work process and that enable sensitive rare cell analysis. To learn more about comprehensive flow cytometry solutions, request the presentation (pdf) or watch the webinar recording (Jul 2011).

The CliniMACS Prodigy® Customized Application Service

The CliniMACS Prodigy® is a single benchtop instrument and offers a variety of pre-installed automated and GMP-compliant cell manufacturing procedures. In cases, where these processes may not fully match user requirements, Miltenyi Biotec offers the translation of individual cell processes into tailor-made applications on the CliniMACS Prodigy®. This webinar (November 2017) introduces the customized application service and the benefits of fully automated cell processing in a closed system. Request the presentation (pdf) or watch the recording .

GMP 101 – Introduction to GMP basics
In the first webinar of the “GMP for translational research” series we will give an introduction to the basic principles of good manufacturing practice (GMP), relevant international norms and an introduction to GMP manufacturing of advanced therapies medicinal products (ATMP). Request the presentation (pdf) or watch the recording (April 2016).

MACSPlex Cytokine – Learn how to simplify your cytokine measurement
MACSPlex Cytokine assays analyze a large number of cells in a very short timeframe and yield highly significant statistical data in terms of cytokine expression and secretion. Lern more about MACSPlex Cytokine assay setup, instrument setup, and the proper use of standards. Furthermore, we will introduce you to the automated analysis features of our MACSPlex Cytokine Express modes. Request the presentation (pdf) or watch the webinar recording (Jun 2015). Presenter: Katrin Lange, R&D, Miltenyi Biotec GmbH

The autoMACS® Pro Separator – the gold standard in walkaway cell separation
With thousands of instruments in use worldwide, the autoMACS Pro Separator provides standardized walkaway cell isolation that is gentle to your cells. In this webinar (March 2014) we explain how to achieve automated multisample cell labeling and separation of viable cells. Using examples from several applications, including whole blood procedures in multiple myeloma and chimerism studies, the versatility of the instrument will be demonstrated. Request the presentation or watch the recording.

Successful cell analysis – optimize the basic steps
Join Dr. Frank Hardung in this webinar (February 2013) on easy preparation of cell samples, magnetic labeling and separation of cells, and quality control of cell separations. Optimizing these basic steps is a prerequisite to successful cell analysis. Request the presentation or watch the recording.

VitalVirus HIV Isolation – How to catch an infectious virus
Do you want to isolate infectious HIV from patient samples and generate primary isolates for downstream experiments? Miltenyi Biotec has developed the VitalVirus HIV Isolation Kits for exactly this purpose. In combination with an optimized PBMC stimulation protocol, infectious HIV can be isolated from patient samples with unrivaled efficiency: on average, 1 in 1,400 virions is infectious. Please request the presentation (pdf) of this webinar (Dec2009).

Viscover

In vivo micro-computed tomography for the time-lapsed evaluation of angiogenesis
Angiogenesis or neovascularization is the early mechanism responsible for the formation of new blood vessels in situations of tissue adaptation and regeneration. Monitoring the growth of neo-vasculature within bio-engineered implants is a valuable tool to assess their integration and functionality. Laura Nebuloni from the ETH Zürich, Switzerland, has developed a new vascular imaging method based on in vivo micro-computed tomography (micro-CT). Request the presentation or watch the recording of this webinar (March 2013) to see how angiogenesis is detected using this micro-CT-based approach in vivo in an ischemia reperfusion situation, as well as in a pre-clinical model for muscle repair.

High resolution and quantitative CT imaging in small animals
See in this webinar how different researchers realized high resolution small animal imaging and quantification with nanoparticulated contrast agents in computed tomography (CT). Examples of scientific CT imaging data will demonstrate how these novel nanoparticles help extend the application range of any micro-CT, far beyond mere bone scans. Request the presentation (pdf) or watch the recording of this webinar by Dr. Raif Yücel, Global Product Manager Viscover™ Preclinical Imaging (April 2012).

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