Research areas - Stem cell research - Mesenchymal stem cells - Cell separation

Target MSC populations with accuracy

  • Reliable: less variation with defined starting populations
  • Superior performance: higher cell proliferation rates compared to conventional plastic adherence
  • Innovative: MSC isolation directly from tissue, without further culture
To investigate the therapeutic potential of mesenchymal stem cells it is crucial to establish efficient and reproducible procedures to obtain MSC populations. Miltenyi Biotec is a long-standing provider of high-quality kits and reagents for the separation of MSCs from different species using a variety of markers.

Human cells

Isolate MSCs from various tissues with ease using MACS® Technology. Be assured that isolated MSC populations retain their high proliferation and differentiation potential in vitro. For example, MSCs separated with the CD271 MicroBead Kit display a 50% higher proliferative capacity and result in 200-fold higher numbers of CFU-Fs than cells isolated by plastic adherence alone.

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Sorting strategies for mouse MSCs

A wide range of MicroBeads is available for the enrichment or purification of mouse MSCs. Various approaches for the separation of MSCs from other cells can be utilized:

  • Contaminating cells can be depleted from mouse MSC cultures by the use of CD11b MicroBeads or a combination of CD45 and Anti-Ter-119 MicroBeads
  • Mouse MSCs expanded from bone marrow cultures can be enriched by selection with CD105, CD117, or Anti-Sca-1 MicroBeads
  • Multipotent plastic-adherent fetal stem cells can be enriched from amniocentesis cultures according to their expression of CD117

Any cell type and any species

Are you utilizing a large animal experimental model? Expand your possibilities with the CD271 or Anti-MSCA-1 antibodies, which cross-react with non-human primate, sheep, swine, canine, and goat MSCs. If the reagent of your interest is not included in the MACS® Cell Separation Reagent portfolio, you can simply use indirect magnetic labeling for customized cell separation. Indirect magnetic labeling is a convenient alternative to direct labeling as any primary antibody may be used for efficient MACS Separation. Discover full experimental flexibility with indirect magnetic labeling.
Find products in Cell separation
21 products available
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Anti-Fibroblast MicroBeads, human

Capacity: for 1×109 total cells
 
130-050-601 $640.00

Anti-MSCA-1 (W8B2) MicroBead Kit, human

Capacity: for 1×109 total cells
 
130-093-583 $615.00

Anti-Sca-1 MicroBead Kit (FITC), mouse

Capacity: for 1×109 total cells
 
130-092-529 $615.00

Anti-Ter-119 MicroBeads, mouse

Capacity: for 2×109 total cells
 
130-049-901 $650.00

CD11b MicroBeads, human and mouse

Capacity: for 1×109 total human cells or for 2×109 total mouse cells
 
130-049-601 $650.00

CD11b MicroBeads, human and mouse – lyophilized

Capacity: for 1×109 total human cells or for 2×109 total mouse cells
 
130-097-142 $650.00

CD45 MicroBeads, human

Capacity: for 1×109 total cells
 
130-045-801 $655.00

CD45 MicroBeads, mouse

Capacity: for 2×109 total cells
 
130-052-301 $640.00

CD45 MicroBeads, mouse – lyophilized

Capacity: for 2×109 total cells
 
130-097-153 $640.00

CD105 MicroBeads, human

Capacity: for 1×109 total cells
 
130-051-201 $650.00
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Showing 1-10 of 21


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