Research areas - Stem cell research - ES and iPS cells - Cell separation

Easily get the cell population you need

  • Fast: well-defined, homogeneous cell populations in less than an hour
  • Flexible: manual and automated sorting, scalable to serve your needs
  • Fully compatible with clinical research requirements:
    no need for transgenic selection markers
To unravel the molecular mechanisms that govern reprogramming, self-renewal, and differentiation of embryonic stem (ES) and induced pluripotent (iPS) stem cells, it is imperative to work with cell populations. We provide a variety of reagents and kits for the gentle and reliable sorting of ES and iPS cells.

Removal of feeder cells

ES and iPS cells are often cultured in the presence of supportive feeder cells that can interfere with downstream applications. Various reagents are available for rapid isolation of ES or iPS cells from feeder cocultures by positive selection or depletion.

 

Human cells

Mouse cells

Read on:

Scientific poster on feeder removal, as presented at ISSCR 2010.

Pluripotent stem cells

ES and iPS cells tend to differentiate in culture. However, for many studies, e.g., differentiation, gene expression, or microRNA analyses, it is crucial to work with pluripotent cultures. We provide the tools for quick and easy isolation of pluripotent stem cells. Isolation is achieved either by depletion of differentiated cells or by positive selection using pluripotency-associated markers, e.g., CD326, CD133, SSEA-3, SSEA-4, TRA-1-60, or TRA-1-81.

Human cells

Mouse cells

Read on:

Report by E. Dick et al. on isolation of TRA-1-81+ iPS cells

Scientific poster on removal of early differentiated cells

Combined removal of feeder and differentiated cells prior to blastocyst
 

ES and iPSC-derived cells

ES and iPS cells can differentiate in vitro into virtually any cell type. MACS® Technology allows the rapid isolation of cells from specific lineages at any time point during differentiation. MACS Technology can be used to deplete unwanted cells during the differentiation process or to enrich the cells of interest by positive selection.

Examples of markers for ES– or iPS cell–derived cell types include:

If the cell marker of your interest is not listed, please use the product finder below or discover how to isolate any cell type from any species.

Read on:

Scientific report on enhanced peripheral neuron differentiation

Customer report by Bai and Wang on ESC-derived vascular progenitors

Application Note on differentiation and enrichment of Neural Crest Stem Cells from human ES/iPSC cultures 

Find products in Cell separation
41 products available
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Showing 1-10 of 41
Product Order no. Price

Anti-A2B5 MicroBead Kit, human and mouse

Capacity: for 1×109 total cells
 
130-097-864 $600.00

Anti-Fibroblast MicroBeads, human

Capacity: for 1×109 total cells
 
130-050-601 $640.00

Anti-MSCA-1 (W8B2) MicroBead Kit, human

Capacity: for 1×109 total cells
 
130-093-583 $615.00

Anti-Prominin-1 MicroBeads, mouse

Capacity: for 5×108 total cells
 
130-092-333 $435.00

Anti-Prominin-1 MicroBeads, mouse – small size

Capacity: for 5×107 total cells
 
130-092-564 $75.00

Anti-PSA-NCAM MicroBead Kit, human and mouse

Capacity: for 1×109 total cells
 
130-097-859 $620.00

Anti-Sca-1 MicroBead Kit (FITC), mouse

Capacity: for 1×109 total cells
 
130-092-529 $615.00

Anti-SSEA-1 (CD15) MicroBeads, human and mouse

Capacity: for 1×109 total cells
 
130-094-530 $490.00

Anti-SSEA-4 MicroBeads, human

Capacity: for 1×109 total cells
 
130-097-855 $490.00

Anti-TRA-1-60 MicroBead Kit, human

Capacity: for 2×108 total cells
 
130-095-816 $615.00
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Showing 1-10 of 41


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