MACS PICK UP POINT Technology
Removal of feeder cells
Pluripotent stem cells
ES and iPSC-derived cells
ES and iPS cells are often cultured in the presence of supportive feeder cells that can interfere with downstream applications. Various reagents are available for rapid isolation of ES or iPS cells from feeder cocultures by positive selection or depletion.
Scientific poster on feeder removal, as presented at ISSCR 2010.
ES and iPS cells tend to differentiate in culture. However, for many studies, e.g., differentiation, gene expression, or microRNA analyses, it is crucial to work with pluripotent cultures. We provide the tools for quick and easy isolation of pluripotent stem cells. Isolation is achieved either by depletion of differentiated cells or by positive selection using pluripotency-associated markers, e.g., CD326, CD133, SSEA-3, SSEA-4, TRA-1-60, or TRA-1-81.
Report by E. Dick et al. on isolation of TRA-1-81+ iPS cells
Scientific poster on removal of early differentiated cells
Combined removal of feeder and differentiated cells prior to blastocyst
Examples of markers for ES– or iPS cell–derived cell types include:
If the cell marker of your interest is not listed, please use the product finder below or discover how to isolate any cell type from any species.
Scientific report on enhanced peripheral neuron differentiation
Customer report by Bai and Wang on ESC-derived vascular progenitors
Application Note on differentiation and enrichment of Neural Crest Stem Cells from human ES/iPSC cultures
Anti-A2B5 MicroBead Kit, human and mouse
Anti-Fibroblast MicroBeads, human
Anti-MSCA-1 (W8B2) MicroBead Kit, human
Anti-Prominin-1 MicroBeads, mouse
Anti-Prominin-1 MicroBeads, mouse – small size
Anti-PSA-NCAM MicroBead Kit, human and mouse
Anti-Sca-1 MicroBead Kit (FITC), mouse
Anti-SSEA-1 (CD15) MicroBeads, human and mouse
Anti-SSEA-4 MicroBeads, human
Anti-TRA-1-60 MicroBead Kit, human
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