Research areas - Neuroscience - Sample preparation - Tissue dissociation - Adult Brain Dissociation Kit, mouse and rat

Adult Brain Dissociation Kit, mouse and rat

Overview

The Adult Brain Dissociation Kit has been designed for the dissociation of rodent neural tissue older than P7 for the subsequent isolation of neurons, astrocytes, or oligodendrocytes.

Details

Background information

Brain tissue from adult mice or rats older than P7 is dissociated into single-cell suspensions. The extracellular matrix is enzymatically digested using the kit components, while the gentleMACS™ Dissociator with Heaters is used for the mechanical dissociation steps during the on-instrument enzyme incubation. After the dissociation, the Debris Removal Solution is used for the elimination of debris, and the Red Blood Cell Removal Solution is used for the removal of erythrocytes.
The single-cell suspension may be analyzed for phenotype distributions, and other functional, genetic, or proteomic studies can be performed. Furthermore, dissociated cells can be cultured, or neurons, astrocytes, or oligodendrocytes can be isolated using MACS® Technology.

Miltenyi TV

Video 1

Immediate delivery
How to isolate pure viable astrocytes

How to isolate pure viable astrocytes

Watch this hands-on protocol for the isolation of astrocytes from mouse brain tissue. The Miltenyi Biotec protocol yields highly purified cells with preserved vitality and can be finished within half a day.

Related Items

Product Order no. Price

Adult Brain Dissociation Kit, mouse and rat

Capacity: for 50 preparations
 
Data sheet
130-107-677 $1,065.00

Customer login

Go

Forgot password?
Register for account

0 Shopping cart

Contact

Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 877 591 1060
macs@miltenyibiotec.com
Contact overview

Product finder

Antibody panel builder

Toolbox

Loading...

Join neural cell course
Join neural cell course

Join neural cell course

Neuro_Contact-Us_Teaser

Contact us

Change country

Please select your country to continue