Myelin Removal Beads II have been developed for the specific removal of myelin debris from single-cell suspensions. Myelin Removal Beads II work without determination of cell number or myelin debris/cell ratio.
When myelin-containing neural tissue is dissociated, large quantities of myelin debris are generated, which considerably impair antibody binding.1,2
Depletion of myelin debris from single-cell suspensions leads to improved immunostaining and cell separation results.
LS or autoMACS® Columns.
Mouse brains (P28) were dissociated using the Neural Tissue Dissociation Kit (P). Flow cytometric analysis shows that the resulting single-cell suspension contains large amounts of myelin membrane fragments and other cellular debris. Only 3% of the signals represent cells (A). Myelin Removal Beads II used with an LS Column and a MidiMACS Separator effectively remove myelin debris (B).
How to isolate pure viable microglia
Watch this hands-on protocol showing you how to isolate microglial cells from adult mouse brain. The Miltenyi Biotec protocol is faster, standardized, reproducible, and easy to scale up.