Research areas - Neuroscience - Sample preparation - Myelin removal - Myelin Removal Beads II, human, mouse, rat

Myelin Removal Beads II, human, mouse, rat

Overview

Myelin Removal Beads II have been developed for the specific removal of myelin debris from single-cell suspensions. Myelin Removal Beads II work without determination of cell number or myelin debris/cell ratio.

Details

Background information

When myelin-containing neural tissue is dissociated, large quantities of myelin debris are generated, which considerably impair antibody binding.1,2
Depletion of myelin debris from single-cell suspensions leads to improved immunostaining and cell separation results.

Columns

LS or autoMACS® Columns.

Gallery

Figure 1

Mouse brains (P28) were dissociated using the Neural Tissue Dissociation Kit (P). Flow cytometric analysis shows that the resulting single-cell suspension contains large amounts of myelin membrane fragments and other cellular debris. Only 3% of the signals represent cells (A). Myelin Removal Beads II used with an LS Column and a MidiMACS Separator effectively remove myelin debris (B).
A:
B:

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Selected references

  1. Pfenninger, C. V. et al. (2007) CD133 is not present on neurogenic astrocytes in the adult subventricular zone, but on embryonic neural stem cells, ependymal cells, and glioblastoma cells. Cancer Res. 67: 5727–5736.
  2. Tham, C. S. et al. (2003) Microglial activation state and lysophospholipid acid receptor expression. Int. J. Dev. Neurosci. 21: 431–443.

Brochures and posters

Neuroscience research brochure [PDF, 1.9 MB, EN]

Scientific posters

Product Order no. Price

Myelin Removal Beads II, human, mouse, rat – small size

Content: 200 µL
 
Data sheet
130-096-731 $100.00

Myelin Removal Beads II, human, mouse, rat

Content: 4 mL
 
Data sheet
130-096-733 $570.00

Myelin Removal Beads II, human, mouse, rat

Content: 2×4 mL
 
Data sheet
130-096-433 $1,005.00

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macs@miltenyibiotec.com
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