- MACSQuant® VYB

MACSQuant® VYB

  • Yellow, violet, and blue lasers, with 10 optical channels for multiparameter flow cytometry
  • Simultaneous immunophenotyping and fluorescent protein analysis
  • Spatially separated lasers to minimize FITC and PE compensation

Overview

Our yellow laser instrument: The MACSQuant® VYB
The MACSQuant VYB gives you all the performance, automation, and convenience of the MACSQuant Analyzer plus an optical layout configured with a yellow laser. As the options in antibodies and fluorochromes continues to expand, you need a flow cytometers that is configured to detect the widest range of colors.
By incorporating a 561nm laser and eight fluorescent channels, the MACSQuant VYB is an ideal choice for analysis using fluorophore-conjugated antibodies as well as fluorescent proteins such as mCherry, DsRed, GFP, CFP and other fruit fluorescent proteins. Additionally, the configuration is optimized to distinguish PE and FITC fluorochromes for improved detection of dim PE populations.

Details

Equipped with three lasers (405, 488, 561 nm), two scatter (FSC, SSC) and eight fluorescent channels, the MACSQuant VYB is the ideal instruments for rapid, fully automated multi-color flow cytometry.
For walk-away processing of up to 96 samples in a single run, enhance your MACSQuant VYB with the optional MACS MiniSampler. The MACS MiniSampler holds tube racks of numerous formats and even microtiter plates, as well up to four reagents for automated staining of cell samples.
The integrated MACSQuant Column enables you to rapidly pre-enrich samples for rare cells prior to flow analysis on the MACSQuant VYB. In just a few simple steps, the entire procedure is complete.
Our broad portfolio of kits and reagents are designed to work together with the MACSQuant VYB to enable efficient identification major cells types, such as dendritic cells, endothelial progenitor cells, cancer stem cells, cytokine-secreting cells, antigen-specific T cells, circulating tumor cells.
Absolute cell counts are determined volumetrically, calculated automatically, and read out as events/μL for every sample and population. Cell counts are available in under a minute, eliminating the need for expensive counting beads or particles.
At the click of a button, you’ll have worldwide, 24 hour remote support from technical support at your finger tip. Our Live Support service allows the user to directly interact with the MACSQuant Application Specialist in real time. Your dedicated support specialist can review your software setup and take control of the instrument to resolve any issues if desired.

Technical specifications

Technical specifications
Optical configuration of the MACSQuant VYB.
Optical configuration of the MACSQuant VYB.

Gallery

Figure 1

The MACSQuant VYB

Figure 2

Figure 2
The MACSQuant VYB can detect a wide range of fluorescent proteins. Histograms of CHO cells transfected with CFP, GFP, YFP (top row), mCherry, and mKate2 (bottom row) are easily distinguished from non‑transfected cells (shown in purple). GFP and YFP are often difficult to distinguish using flow cytometry due to similarities in their emission spectra. With the MACSQuant VYB, YFP‑ (shown in yellow) and GFP‑expressing cells (shown in green) are clearly distinguishable when observed in the PI channel (bottom right), making it possible to detect up to five fluorescent proteins in a single sample.
The MACSQuant VYB can detect a wide range of fluorescent proteins. Histograms of CHO cells transfected with CFP, GFP, YFP (top row), mCherry, and mKate2 (bottom row) are easily distinguished from non‑transfected cells (shown in purple). GFP and YFP are often difficult to distinguish using flow cytometry due to similarities in their emission spectra. With the MACSQuant VYB, YFP‑ (shown in yellow) and GFP‑expressing cells (shown in green) are clearly distinguishable when observed in the PI channel (bottom right), making it possible to detect up to five fluorescent proteins in a single sample.

Figure 3

Figure 3
Improved detection of PE fluorescence with the MACSQuant VYB Cells expressing GFP under an Oct4 promoter and labeled with Anti‑Feeder-PE, a dim fibroblast marker, were analyzed using the MACSQuant VYB. Excitation of both PE and GFP with the 488 nm blue laser only minimally resolves the two signals (A). In comparison, excitation of PE by the yellow 561 nm laser and of GFP by the blue 488 nm laser fully resolved these two signals without the need for any compensation (B).
Improved detection of PE fluorescence with the MACSQuant VYB Cells expressing GFP under an Oct4 promoter and labeled with Anti‑Feeder-PE, a dim fibroblast marker, were analyzed using the MACSQuant VYB. Excitation of both PE and GFP with the 488 nm blue laser only minimally resolves the two signals (A). In comparison, excitation of PE by the yellow 561 nm laser and of GFP by the blue 488 nm laser fully resolved these two signals without the need for any compensation (B).

Figure 4

Figure 4
APC staining with the MACSQuant VYB. B cells were stained with CD19‑PE and CD20‑APC and analyzed by using the MACSQuant VYB (left). Lymphocytes were stained with CD8‑APC and analyzed with the MACSQuant Analyzer 10 (middle) and the MACSQuant VYB (right). APC resolution with the yellow 561 nm laser of the MACSQuant VYB is equal to the resolution with the red 635 nm laser of the MACSQuant Analyzer 10. MFI: mean fluorescence intensity, SI: stain index.
APC staining with the MACSQuant VYB. B cells were stained with CD19‑PE and CD20‑APC and analyzed by using the MACSQuant VYB (left). Lymphocytes were stained with CD8‑APC and analyzed with the MACSQuant Analyzer 10 (middle) and the MACSQuant VYB (right). APC resolution with the yellow 561 nm laser of the MACSQuant VYB is equal to the resolution with the red 635 nm laser of the MACSQuant Analyzer 10. MFI: mean fluorescence intensity, SI: stain index.

Figure 5

Figure 5
Minimal compensation between the two most popular dyes used in flow cytometry: FITC and PE. Cells expressing GFP under an Oct4 promoter were labeled with Anti-Feeder-PE, a dim fibroblast marker. When FITC/GFP and PE are analyzed with the MACSQuant VYB (B), minimal or no compensation is needed as FITC/GFP and PE are detected using the 488 nm blue laser and the 561 nm yellow laser, respectively. In contrast, when both FITC/GFP and PE are analyzed using the 488 nm blue laser (A), major compensation is needed. All files displayed with no compensation applied.
Minimal compensation between the two most popular dyes used in flow cytometry: FITC and PE. Cells expressing GFP under an Oct4 promoter were labeled with Anti-Feeder-PE, a dim fibroblast marker. When FITC/GFP and PE are analyzed with the MACSQuant VYB (B), minimal or no compensation is needed as FITC/GFP and PE are detected using the 488 nm blue laser and the 561 nm yellow laser, respectively. In contrast, when both FITC/GFP and PE are analyzed using the 488 nm blue laser (A), major compensation is needed. All files displayed with no compensation applied.

Figure 6

Figure 6
Multicolor phenotyping: benefit from the MACSQuant VYB’s full potential. Perform classic immunophenotyping with the MACSQuant VYB. Shown here is a 6‑color analysis of PBMCs with a panel of standard antibodies: CD45‑VioBlue, CD3‑FITC, CD4‑PE, CD8‑APC, CD45RA‑APC‑Cy7, and PI.
Multicolor phenotyping: benefit from the MACSQuant VYB’s full potential. Perform classic immunophenotyping with the MACSQuant VYB. Shown here is a 6‑color analysis of PBMCs with a panel of standard antibodies: CD45‑VioBlue, CD3‑FITC, CD4‑PE, CD8‑APC, CD45RA‑APC‑Cy7, and PI.

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MACSQuant VYB

Content: 1 unit
 
User manual
130-096-116 price on request here

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Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 877 591 1060
macs@miltenyibiotec.com
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