Research areas - Immunology - Autoimmunity - Psoriasis

Psoriasis

Psoriasis is a chronic systemic inflammatory disorder resulting from a combination of predisposing genetic factors and environmental triggers. It is a hereditary disease elicited by chronic activation of cutaneous T cells. Disease manifestation is orchestrated by pro-inflammatory CD4+ T helper cells producing either IFN-γ (Th1 cells) or IL-17 (Th17 cells). These cells interact with dermal resident dendritic cells, macrophages, mast cells, and neutrophils.

Download a list of selected references on psoriasis research.

Sample preparation

Simplify and standardize the dissociation of tissue samples by using the gentleMACS Dissociators in combination with Tissue Dissociation Kits. Retrieve maximum amounts of viable cells from, e.g., mouse epidermis using the Epidermis Dissociation Kit.

Cell separation

Psoriatic epidermis and dermis contain leukocytic infiltrates, including neutrophils, dendritic cells, and T cells. Miltenyi Biotec offers a wide range of cell separation products to isolate these target cell populations with ease, e.g. dendritic cells or neutrophils. The Epidermal Langerhans Cell MicroBead Kit allows the isolation of mouse Langerhans cells from epidermal single-cell suspensions.

Cell analysis

Miltenyi Biotec provides a large set of tools for multicolor flow cytometry suitable for the identification and characterization of inflammatory cell types involved in psoriasis. Epidermis-resident cells, such as CD11c+CD207+ Langerhans cells can be reliably detected and characterized using Miltenyi Biotec’s high-quality antibodies.
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autoMACS Pro Separator - Starter Kit

Components:
  • autoMACS Pro Separator Instrument
  • MACS MiniSampler
  • MACS Reagent Rack 4 (130-094-574)
  • Chill 5, 15, 50 Rack Set (130-097-038)
  • barcode reader
  • software
  • autoMACS® Columns (130-021-101)
  • 4×column substitute
  • autoMACS Pro Buffer Combination
  • User manual
  • short instructions
  • one-year warranty
 
130-092-545 price on request here

MACSQuant Analyzer 10

Content: 1 unit
 
130-096-343 price on request here

MACSQuant VYB

Content: 1 unit
 
130-096-116 price on request here

MultiMACS Cell24 Separator Plus

Components:
  • MultiMACS Cell24 Separator
  • MACS Elution Station
  • Elution Chamber Base
  • Elution Chamber Lid I
  • Elution Chamber Lid II
  • Multi-24 Column Block (130-095-692)
  • 2×24-well Deep Well Plate
  • Single-Column Adapter
  • MultiMACS 5 mL Tube Rack (130-095-331)
  • Column Blank Holder and 9 Column Blanks
  • Tubing
  • Power cord
  • Null modem cable
  • High power CAN cable
 
130-098-637 price on request here

21 CFR Part 11 enabled MACSQuantify Software V2.11, additional PC license

130-115-387 price on request here

21 CFR Part 11 enabled MACSQuantify Software V2.11, initial setup

160-002-213 price on request here

24-well Deep Well Plates

Content: 4 pieces (1)

Packing unit

(1)sterile packaged
 
130-110-500 $45.00

Adipose Tissue Progenitor Isolation Kit, mouse

Capacity: for up to 1×108 total cells
 
130-106-639 $435.00

Annexin V MicroBead Kit

Capacity: for 1×109 total cells
 
130-090-201 $700.00

Anti-BP-1 (Ly-51)-FITC, mouse

For research use only
Content: 30 µg in 1 mL (1)

Quantity

(1)200 tests. One test corresponds to labeling of up to 106 cells in a final volume of 50 µL.
 
130-102-170 $40.00
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