Research areas - Cardiovascular research - Tissue derived cardiac cells - Cell separation

Isolate pure cardiac cell types in a heartbeat

  • Homogeneous cell populations in less than one hour
  • No sequential plating of isolated cells needed
  • Highly reproducible purities and recoveries

MACS® Technology enables fast, efficient, and highly reproducible enrichment of all three major cardiac cell types of the neonatal heart as well as fibroblasts and endothelial cells from adult cardiac tissue. Simply incubate the single cell suspension from the dissociated hearts with the specific MACS MicroBead, pass the labeled cells through a magnetic field of a MACS Separator, and elute your cell type of interest.

Whether you process one or multiple samples at a time, Miltenyi Biotec has the right solution for your lab’s throughput. MACS® Technology relies on 3 components:

  • MACS MicroBeads – superparamagnetic particles conjugated to a highly specific, monoclonal antibody against a cell surface antigen
  • MACS Columns – contain a matrix composed of ferromagnetic spheres covered with a cell-friendly coating
  • MACS Separators – for manual or automated solutions - contain powerful permanent magnets that induce a high-gradient magnetic field within MACS Columns – a field strong enough to retain cells labeled with even small amounts of MACS MicroBeads
For more information, visit www.miltenyibiotec.com/cellseparation

Cardiomyocyte isolation

Cardiomyocytes were isolated from dissociated rat and mouse hearts (P0-P3) using the respective Neonatal Cardiomyocyte Isolation Kit. The immunomagnetic isolation resulted in high yields of viable cardiomyocytes with purities of up to 98%, as shown for rat samples in figure 1A and rat and mouse samples in figure 1B. Immunofluorescent stainings of mouse cells were performed before (1C) and after (1D) cardiomyocyte isolation. Cells were stained for α-actinin(green) and vimentin (red). The blue stain (DAPI) denotes the nuclei.

Cardiac fibroblast isolation

Cardiac fibroblast were isolated from dissociated rat and mouse hearts (P0-P3) using the respective Neonatal Cardiomyocyte Isolation Kit. The isolation yielded high recovery rates of viable cardiac fibroblast with purities of up to 96% (1A). Immunofluorescent stainings of mouse cells were performed before (1B) and after (1C) cardiac fibroblast isolation. Cells were stained for α-actinin(green) and vimentin (red). The blue stain (DAPI) denotes the nuclei.
 

Cardiac endothelial cell isolation

Cardiac endothelial cells were isolated from dissociated mouse hearts (P0-P3) using the Neonatal Cardiomyocyte Isolation Kit. The isolation yielded high recovery rates of viable cardiac endothelial cells with purities of up to 97% (1A,B). Isolated cardiac endothelial cells maintained their characteristic morphology upon isolation (1C). Moreover, the functionality of enriched endothelial cell was proven by the uptake of Dil-Ac-LDL (red) (1D). The blue stain (DAPI) denotes the nuclei.
 
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Cardiac Progenitor Cell Isolation Kit (Sca-1), mouse

Capacity: for up to 100 separations
 
130-098-374 $710.00

CD31 MicroBeads, mouse

Capacity: for 2×109 total cells
 
130-097-418 $640.00

CD90.1 MicroBeads, mouse and rat

Capacity: for 2×109 total cells
 
130-094-523 $640.00

CD90.2 MicroBeads, mouse

Capacity: for 2×109 total cells
 
130-049-101 $640.00

CD90.2 MicroBeads, mouse – lyophilized

Capacity: for 2×109 total cells
 
130-097-147 $640.00

CD117 MicroBeads, mouse

Capacity: for 1×109 total cells
 
130-091-224 $555.00

CD117 MicroBeads, mouse – lyophilized

Capacity: for 1×109 total cells
 
130-097-146 $555.00

Neonatal Cardiac Endothelial Cell Isolation Kit, mouse

Capacity: for up to 2.5×108 total cells
 
130-104-183 $410.00

Neonatal Cardiac Endothelial Cell Isolation Kit, rat

Capacity: for up to 5×108 total cells
 
130-106-768 $400.00

Neonatal Cardiac Fibroblast Isolation Kit, mouse

Capacity: for up to 5×108 total cells
 
130-101-372 $720.00
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Phone: +1 800 FOR MACS
Fax:+1 877 591 1060
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