Differentiation of ES/iPS cells into cardiac cells is a well-established system to study cardiac development or to produce different cardiac cell types for various applications. Now, the MACS Technology
facilitates the isolation of pure cardiovascular progenitor cells from such cultures, based on the cells’ CD309 (Flk-1) expression. CD309 is one of the earliest differentiation markers for endothelial and hematopoietic cells. Using mouse embryonic stem cells, it has been shown that CD309 is a marker for multipotent cardiovascular progenitor cells that give rise to cardiomyocyte, endothelial, and vascular smooth muscle cell lineages.
Moreover, use our CD144 MicroBeads for the isolation of human iPS cell derived endothelial cells and our CD184 MicroBeads for the isolation of human iPS cell derived endodermal cells.
Whether you process one or multiple samples at a time, Miltenyi Biotec has the right solution for your lab’s throughput.
MACS Technology relies on 3 components:
- MACS MicroBeads – superparamagnetic particles conjugated to a highly specific, monoclonal antibody against a cell surface antigen
- MACS Columns – contain a matrix composed of ferromagnetic spheres covered with a cell-friendly coating
- MACS Separators – for manual or automated solutions - contain powerful permanent magnets that induce a high-gradient magnetic field within MACS Columns – a field strong enough to retain cells labeled with even small amounts of MACS MicroBeads
For more information, visit www.miltenyibiotec.com/cellseparation