Research areas - Cardiovascular research - ES/iPS derived cardiac cells - Cell analysis - Anti-α-Actinin (Sarcomeric) antibodies, human, mouse, rat

Anti-α-Actinin (Sarcomeric) antibodies, human, mouse, rat (clone: REA402)

Overview

Clone REA402 recognizes the sarcomeric α-actinin antigen, a 100 kDa actin-binding protein, which occupies a strategic role in the assembly and maintenance of stress fibers of non-muscle cells and the myofibrils of muscle cells. α-actinins have been highly conserved throughout evolution and are largely collinear proteins that share three conserved functional domains. Functionally, α-actinins form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. Two isoforms of α-actinin have been identified, a muscle-specific sarcomeric isoform and a non-sarcomeric isoform. The major – and best characterized – functional difference among α-actinin isoforms is calcium sensitivity for actin-binding. The binding of α-actinin to actin by non-muscle cytoskeletal isoforms is dependent on the calcium concentration, whereas this interaction is independent of calcium concentration in sarcomeric striated and smooth muscle isoforms. Studies on skeletal and cardiac cells have localized sarcomeric α-actinin to Z-bands in striated myofibrils, to precursor I-Z-I-like complexes in muscle undergoing myofibrillogenesis, and to vinculin-positive adhesion plaques and adherens junctions.
Additional information: Clone REA402 displays negligible binding to Fc receptors.

Technical data


Antigen α-Actinin
Clone REA402
Isotype recombinant human IgG1
Isotype control REA Control (I) antibodies
Alternative names of antigen ACTN2, CMD1AA, CMH23
Entrez Gene ID 88
Molecular mass of antigen [kDa] 104
Cross-reactivity rhesus monkey (Macaca mulatta), cynomolgus monkey (Macaca fascicularis), sheep, rabbit, lizard, feline, canine, snake, fish, goat, chicken, bovine, frog, pig, hamster
Distribution of antigen ES and iPS cells, other
Endotoxin level
Product format Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Fixation The antibody is suited for staining of formaldehyde-fixed cells.
Storage Store protected from light at 2–8 °C. Do not freeze.
Available conjugates FITC, PE, VioBlue, Biotin, pure

Gallery

Figure 1

Heart tissue from P1 Wistar rats was dissociated using the Neonatal Heart Dissociation Kit and the gentleMACS™ Dissociator. Neonatal cardiomyocytes were then fixed, permeabilized, and stained with Anti-α-Actinin (Sarcomeric) antibodies or with the corresponding REA Control antibodies (left image). Flow cytometry was performed using the MACSQuant® Analyzer. Cell debris were excluded from the analysis based on scatter signals.

Related Items

Library

Selected references

  1. Lu, M. H. et al. (1992) The vinculin/sarcomeric-alpha-actinin/alpha-actin nexus in cultured cardiac myocytes. J. Cell Biol. 117(5): 1007–1022.
  2. Young, P. et al. (1998) Molecular structure of the sarcomeric Z-disk: two types of titin interactions lead to an asymmetrical sorting of alpha-actinin. EMBO J. 17(6): 1614–1624.
  3. Zhang, Z. Q. et al. (2009) Sarcomeric-alpha-actinin defective in vinculin-binding causes Z-line expansion and nemaline-like body formation in cultured chick myotubes. Exp. Cell Res. 315(5): 748–759.

Customer reports and application notes

Special protocols

Product Order no. Price

Anti-α-Actinin (Sarcomeric)-FITC, human, mouse, rat (clone: REA402)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-106-997 $80.00

Anti-α-Actinin (Sarcomeric)-FITC, human, mouse, rat (clone: REA402)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-106-936 $225.00

Anti-α-Actinin (Sarcomeric)-PE, human, mouse, rat (clone: REA402)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-106-998 $100.00

Anti-α-Actinin (Sarcomeric)-PE, human, mouse, rat (clone: REA402)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-106-937 $270.00

Anti-α-Actinin (Sarcomeric)-VioBlue, human, mouse, rat (clone: REA402)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-106-996 $120.00

Anti-α-Actinin (Sarcomeric)-VioBlue, human, mouse, rat (clone: REA402)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-106-935 $290.00

Anti-α-Actinin (Sarcomeric)-Biotin, human, mouse, rat (clone: REA402)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-106-995 $85.00

Anti-α-Actinin (Sarcomeric)-Biotin, human, mouse, rat (clone: REA402)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
Data sheet
130-106-934 $225.00

Anti-α-Actinin (Sarcomeric) pure, human, mouse, rat (clone: REA402)

For research use only
Content: 100 µg in 100 µL
 
Data sheet
130-112-755 $125.00

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Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 877 591 1060
macs@miltenyibiotec.com
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