The analysis of cancer stem cell populations requires effective methods for tissue dissociation. The gentleMACS Dissociators in combination with the different tumor dissociation kits reliably dissociates the extracellular matrix and cell adhesion components of tumor masses while conserving cell surface epitopes to obtain viable single-cell suspensions.
- The Tumor Dissociation Kit, human was developed for the preparation of cancer cells from human primary tumor tissue or metastases. It has been successfully used for the dissociation of numerous human primary tumor tissues as well as xenografted tumors.
- The Tumor Dissociation Kit, mouse was developed for the preparation of cancer cells from mouse tumors.
- The Brain Tumor Dissociation Kit was developed for the gentle yet effective dissociation of human brain tumors.
For certain samples, for example, severely necrotic tumors, the removal of cell debris and dead cells prior to downstream experiments is strongly recommended. Miltenyi Biotec has developed unique reagents, such as the Dead Cell Removal Kit
, to achieve this – with convenience and effectiveness in mind.