Research areas - Application Spotlight - Mo-DC generation

How to generate Mo-DC

The standardized and automated workflow solution for the generation and maturation of human monocyte-derived dendritic cells (Mo-DCs).


  • Complete workflow for Mo-DC generation from basic to clinical research
  • Fully automated cell separation and analysis for routine and large scale Mo-DC generation
  • Ready-to-use and pre-tested cell culture reagents with lot-specific certificates of analysis for high reproducibility
Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research only and not for therapeutic or diagnostic use.

Efficient monocyte isolation

Researchers around the world trust us for their Mo isolation. MACS CD14 Microbeads and the Mo-DC Isolation Kit are powered by MACS Technology, which is cited in over 20,000 publications to date. This proven technology can be fully automated for large-scale and high throughput processing of monocytes using the AutoMACS Pro Separator and MultiMACS Cell24 Separator Plus instruments.


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Differentiation and maturation of Mo-DC

With high quality comes reproducibility for results you can trust. MACS Cytokines give you the best conditions for reproducible Mo-DC cultures, including lot-specific biological activity, low endotoxin, and optimized carrier protein-free formulation. MACS Cytokines for Mo-DC culture and PepTivator peptide pools are available up to GMP-grade. Our ready-to-use media are the popular choice for large-scale production and researchers new to the field. Of course, our qualified technical support is there around the clock to assist you with lot reservations and custom solutions.

Educational resources

Mo-DC phenotyping

The MACSQuant Flow Cytometers and MACS Antibodies were optimized to work together for high-throughput DC phenotyping.
When you need consistent staining with low background, choose our exclusive REAfinity Antibodies, engineered for extraordinary specificity. Analyzing Mo-DC differentiation and maturation? The Mo-DC Differentiation Inspector enables fast and easy evaluation of the in vitro generation of Mo-DCs.


Educational resources

Gentle tissue dissociation for DC priming

Is your focus DC cancer immunotherapy? Then you'll want to try our walk-away tumor dissociation package, which includes the gentleMACS Dissociator and Tumor Dissociation Kit. This winning combination uses standardized programs and protocols to enable unmatched reproducibility and preserve antigen epitopes for DC priming.


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autoMACS Pro Separator - Starter Kit

Components:
  • autoMACS Pro Separator Instrument
  • MACS MiniSampler
  • MACS Reagent Rack 4 (130-094-574)
  • Chill 5, 15, 50 Rack Set (130-097-038)
  • barcode reader
  • software
  • autoMACS® Columns (130-021-101)
  • 4×column substitute
  • autoMACS Pro Buffer Combination
  • User manual
  • short instructions
  • one-year warranty
 
130-092-545 price on request here

CliniMACS Plus Instrument

Availability: Europe (1), USA (2), other (3)
Content: 1 unit

Availability

(1)In the EU, the CliniMACS System components are available as CE-marked medical devices for their respective intended use, unless otherwise stated.
(2)In the US, the CliniMACS CD34 Reagent System, including the CliniMACS Plus Instrument, CliniMACS CD34 Reagent, CliniMACS Tubing Sets TS and LS, and the CliniMACS PBS/EDTA Buffer, is FDA approved. All other products of the CliniMACS Product Line are available for use only under an approved Investigational New Drug (IND) application or Investigational Device Exemption (IDE).
(3)For availability in your country please contact your local representative.
 
151-01 price on request here

CliniMACS Prodigy

Availability: Europe (1), USA (2), other (3)
Content: 1 unit

Availability

(1)In the EU, the CliniMACS System components are available as CE-marked medical devices for their respective intended use, unless otherwise stated.
(2)In the US, the CliniMACS CD34 Reagent System, including the CliniMACS Plus Instrument, CliniMACS CD34 Reagent, CliniMACS Tubing Sets TS and LS, and the CliniMACS PBS/EDTA Buffer, is FDA approved. All other products of the CliniMACS Product Line are available for use only under an approved Investigational New Drug (IND) application or Investigational Device Exemption (IDE).
(3)For availability in your country please contact your local representative.
 
200-075-301 price on request here

gentleMACS Dissociator

Components:
  • gentleMACS Dissociator
  • Power cord
  • User manual
 
130-093-235 price on request here

gentleMACS Octo Dissociator

Components:
  • gentleMACS Octo Dissociator
  • Power cord
  • User manual
 
130-095-937 price on request here

gentleMACS Octo Dissociator with Heaters

Components:
  • gentleMACS Octo Dissociator with Heaters
  • Power cord
  • User manual
 
130-096-427 price on request here

MACSQuant Analyzer 10

Content: 1 unit
 
130-096-343 price on request here

MACSQuant VYB

Content: 1 unit
 
130-096-116 price on request here

MultiMACS Cell24 Separator Plus

Components:
  • MultiMACS Cell24 Separator
  • MACS Elution Station
  • Elution Chamber Base
  • Elution Chamber Lid I
  • Elution Chamber Lid II
  • Multi-24 Column Block (130-095-692)
  • 2×24-well Deep Well Plate
  • Single-Column Adapter
  • MultiMACS 5 mL Tube Rack (130-095-331)
  • Column Blank Holder and 9 Column Blanks
  • Tubing
  • Power cord
  • Null modem cable
  • High power CAN cable
 
130-098-637 price on request here

Anti-HLA-DR-FITC, human (clone: AC122)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
130-098-176 $80.00
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