Research areas - Application Spotlight - Microglia isolation

How to isolate microglia

Why waste two weeks when you could isolate pure microglia in only a few hours?


  • Save time with our 4-hour workflow vs. a 2-week shake-off method
  • Pure single-cell suspensions with conserved antigen epitopes
  • Isolate microglia even from adult tissue

Standardized tissue dissociation

Polarization of T helper cells
How to isolate pure viable microglia
Everything you need for standardized tissue dissociation is here: titrated enzymes, a detailed protocol, and walk-away automation. When harvesting multiple animals, the gentleMACS Octo Dissociator with Heaters enables you to process up to 8 samples in parallel. This gentle yet effective dissociation method preserves vital epitopes for effective downstream applications every time.
Improve your downstream results by removing unwanted myelin from your sample. You'll experience better antibody binding in subsequent analyses and minimize debris under the microscope or in culture. 


Educational resources

Fast isolation of microglia

Polarization of T helper cells
How to isolate pure viable microglia
Why waste 2 weeks using the shake off method, when you could isolate microglia in just 1 hour? Eliminate the unnecessary labor and decrease the risk of modifications of your cells, as well as contamination in culture. Get a higher yield of viable cells when you choose a MACS Column instead of the cell sorter.


Educational resources

Flow cytometry for microglia

Polarization of T helper cells
How to isolate pure viable microglia
Want precise analysis of cell population and numbers? Then flow cytometry is the perfect addition to analysis. Our engineered REAfinity Antibodies provide increased specificity with reliably consistent staining and low background. Choose among a broad panel of specific antibodies and brighter fluorochromes for easy multi-color panel design.


Educational resources

High-quality cytokines

Polarization of T helper cells
How to isolate pure viable microglia
Why compromise on quality when choosing cytokines for your next experiment. MACS Cytokines, including Human and Mouse M-CSF, are manufactured under the highest standards for maximal reproducibility. Available in research and premium grade, our cytokines make transitioning your research to preclinical applications seamless.


Educational resources
Find products in Products & Services
126 products available


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autoMACS Pro Separator - Starter Kit

Components:
  • autoMACS Pro Separator Instrument
  • MACS MiniSampler
  • MACS Reagent Rack 4 (130-094-574)
  • Chill 5, 15, 50 Rack Set (130-097-038)
  • barcode reader
  • software
  • autoMACS® Columns (130-021-101)
  • 4×column substitute
  • autoMACS Pro Buffer Combination
  • User manual
  • short instructions
  • one-year warranty
 
130-092-545 price on request here

gentleMACS Dissociator

Components:
  • gentleMACS Dissociator
  • Power cord
  • User manual
 
130-093-235 price on request here

gentleMACS Octo Dissociator

Components:
  • gentleMACS Octo Dissociator
  • Power cord
  • User manual
 
130-095-937 price on request here

gentleMACS Octo Dissociator with Heaters

Components:
  • gentleMACS Octo Dissociator with Heaters
  • Power cord
  • User manual
 
130-096-427 price on request here

MACSQuant Analyzer 10

Content: 1 unit
 
130-096-343 price on request here

MACSQuant VYB

Content: 1 unit
 
130-096-116 price on request here

MultiMACS Cell24 Separator Plus

Components:
  • MultiMACS Cell24 Separator
  • MACS Elution Station
  • Elution Chamber Base
  • Elution Chamber Lid I
  • Elution Chamber Lid II
  • Multi-24 Column Block (130-095-692)
  • 2×24-well Deep Well Plate
  • Single-Column Adapter
  • MultiMACS 5 mL Tube Rack (130-095-331)
  • Column Blank Holder and 9 Column Blanks
  • Tubing
  • Power cord
  • Null modem cable
  • High power CAN cable
 
130-098-637 price on request here

Brain Tumor Dissociation Kit (P)

Capacity: for 25 preparations
 
130-095-942 $450.00

Brain Tumor Dissociation Kit (T)

Capacity: for 25 preparations
 
130-095-939 $450.00

C Tubes

Content: 25 tubes (1)

Packing unit

(1)sterile, single-packed
 
130-093-237 $150.00
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