Research areas - Application Spotlight - T helper cell polarization

How to polarize T helper cells

The complete solution for your polarizing experiments, optimized for time-savings and publication-quality results.


  • One seamless workflow, optimized by the T cell experts
  • Serum-free medium for standardized cell culture conditions
  • High-quality cytokines up to GMP grade

Gentle tissue dissociation

Start your experiment with a quality sample preparation for the best results. The gentleMACS Dissociator and MACS Tissue Dissociation kits are optimized to work together for gentle and effective dissociation of many tissue types, like lymphoid organs.


Educational resources

Polarization of T helper cells
Polarization of T helper cells

Isolation of pure naive CD4+ T cells

Get publication-quality cells in as little as 18 minutes. Using the Naive CD4 T Cell Isolation Kit, you'll isolate highly pure human or mouse naive CD4+ T cells fast. Automate the process with the autoMACS Pro Separator for even greater convenience and reproducibility. Processing multiple samples in parallel? Then the MultiMACS Cell24 Plus Separator is for you. Isolate cells from up to 24 samples simultaneously.


Educational resources

Polarization of T helper cells
Polarization of T helper cells

Reproducible T cell polarization

You've come so far, so why choose anything but the highest quality cell culture products for naive CD4+ T cells? TexMACS Medium is a serum-free medium for T cell expansion and polarization. Premium grade MACS Cytokines will ensure same unit-dosage for your T cell expansion and polarizing experiments. Eliminate variability in your cell culture conditions and experience faster T cell expansion rates.


Educational resources

Polarization of T helper cells
Polarization of T helper cells

Easy multi-color flow analysis

Look no further to find everything you need for your next flow cytometery experiment. Brighter fluorochromes for multi-color panel designs and engineered REAfinity Antibodies for high specificity and low background staining are just some of the reasons why researchers choose us for their flow cytometery needs. Want to enrich viable cytokine-secreting cells without fixation and detect small numbers of cells efficiently? Try the MACS Cytokine Secretion assays. When fast cytokine detection is what you need, check out the Rapid Cytokine Inspector Kits. Need multiple cytokine detection? Use MACSplex Cytokine Kits.


Educational resources

Polarization of T helper cells
Polarization of T helper cells
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autoMACS Pro Separator - Starter Kit

Components:
  • autoMACS Pro Separator Instrument
  • MACS MiniSampler
  • MACS Reagent Rack 4 (130-094-574)
  • Chill 5, 15, 50 Rack Set (130-097-038)
  • barcode reader
  • software
  • autoMACS® Columns (130-021-101)
  • 4×column substitute
  • autoMACS Pro Buffer Combination
  • User manual
  • short instructions
  • one-year warranty
 
130-092-545 price on request here

gentleMACS Octo Dissociator

Components:
  • gentleMACS Octo Dissociator
  • Power cord
  • User manual
 
130-095-937 price on request here

gentleMACS Octo Dissociator with Heaters

Components:
  • gentleMACS Octo Dissociator with Heaters
  • Power cord
  • User manual
 
130-096-427 price on request here

MACSQuant Analyzer 10

Content: 1 unit
 
130-096-343 price on request here

MultiMACS Cell24 Separator Plus

Components:
  • MultiMACS Cell24 Separator
  • MACS Elution Station
  • Elution Chamber Base
  • Elution Chamber Lid I
  • Elution Chamber Lid II
  • Multi-24 Column Block (130-095-692)
  • 2×24-well Deep Well Plate
  • Single-Column Adapter
  • MultiMACS 5 mL Tube Rack (130-095-331)
  • Column Blank Holder and 9 Column Blanks
  • Tubing
  • Power cord
  • Null modem cable
  • High power CAN cable
 
130-098-637 price on request here

Anti-Bcl-6-PE, human and mouse (clone: REA373)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 106 cells in a total volume of 100 µL.
 
130-105-486 $95.00

Anti-Bcl-6-PE, human and mouse (clone: REA373)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 106 cells in a total volume of 100 µL.
 
130-105-443 $265.00

Anti-Bcl-6-APC, human and mouse (clone: REA373)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 106 cells in a total volume of 100 µL.
 
130-105-485 $120.00

Anti-Bcl-6-APC, human and mouse (clone: REA373)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 106 cells in a total volume of 100 µL.
 
130-105-442 $280.00

Anti-CCR10-PE, human (clone: REA326)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
130-104-868 $95.00
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