Research areas - Application Spotlight - PSC reprogramming

How to reprogram pluripotent stem cells

The optimized workflow for reprogramming, expansion, and maintenance of human pluripotent stem cells (PSCs).

 

  • Reliable and robust media for PSC maintenance culture
  • Fast and efficient mRNA reprogramming
  • Xeno- and serum-free reagents for seamless clinical translation

Gentle fibroblast dissociation

Go from biopsy to fibroblast culture in just a few hours. The gentleMACS Dissociator together with the Whole Skin Dissociation Kit provide the most gentle and efficient way to generate single-cell suspensions from human skin tissue.


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Simple reprogramming and maintenance

Reprogramming
Truly integration-free reprogramming is now available in an easy-to-use system. The StemMACS mRNA Reprogramming Kit enables you to isolate iPS cells in as little as 14 days. Our protocol eliminates the need for feeder cells or conditioned media. All this and you'll perform your reprogramming experiment cost-effectively in a 24-well format.
Maintenance culture
StemMACS iPS-Brew XF is the robust, reliable, and cost-effective PSC culture medium you're looking for. Our xeno- and serum-free media enable you to seamlessly translation your basic research to preclinical applications. Which other media can say that?


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Easy isolation of reprogrammed cells

Bring speed and convenience to your isolation of fully reprogrammed pluripotent cells. Choose TRA-1-60 Microbeads and the MultiMACS Cell24 Separator Plus to isolate cells with the highest purity and viability. This flexible instrument gives you the option to easily transition to high-throughput and integration into automated liquid handling systems. Alternatively, you can use live cell staining in culture to facilitate colony picking. Our live cell stains are azide-free, making them ideal for staining directly in the culture dish. No fixation, no sample loss.


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The complete package for flow cytometry

Here you'll find everything you need to phenotype your reprogrammed iPS cells - fluorochrome-conjugated antibodies, kits, bright dyes, and flow cytometers. Planning a multi-parameter panel? Find the optimal combination of antibodies and dyes using our intuitive panel builder. If you're need consistent staining with the lowest background, create a panel of REAfinity Antibodies. Using colony picking after reprogramming? Keep an eye out for our live cell staining solution, coming soon. And when you can't find the antibody you need, contact our Custom Antibody Design Service to discuss a customized antibody made to order.


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gentleMACS Dissociator

Components:
  • gentleMACS Dissociator
  • Power cord
  • User manual
 
130-093-235 price on request here

MACSQuant Analyzer 10

Content: 1 unit
 
130-096-343 price on request here

Anti-Nanog-PE, human (clone: REA314)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
130-105-080 $95.00

Anti-Nanog-PE, human (clone: REA314)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
130-105-050 $265.00

Anti-Nanog-APC, human (clone: REA314)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
130-105-079 $120.00

Anti-Nanog-APC, human (clone: REA314)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
130-105-049 $280.00

Anti-Nanog pure, human (clone: REA314)

For research use only
Content: 100 µg in 1 mL
 
130-104-732 $150.00

Anti-Oct3/4 Isoform A-PE, human and mouse (clone: REA338)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 106 cells in a total volume of 100 µL.
 
130-105-606 $95.00

Anti-Oct3/4 Isoform A-PE, human and mouse (clone: REA338)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 106 cells in a total volume of 100 µL.
 
130-105-554 $265.00

Anti-Oct3/4 Isoform A-APC, human and mouse (clone: REA338)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 106 cells in a total volume of 100 µL.
 
130-105-607 $120.00
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Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 877 591 1060
macs@miltenyibiotec.com
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