Research areas - Application Spotlight - Neural cell isolation from adult rodent brains

Neural cell isolation from adult rodent brains

We have developed a workflow to investigate adult rodent neural cells that includes adult brain dissociation (>P7), isolation of neurons, astrocytes, microglia, oligodendrocytes, and endothelial cells, cultivation, and flow cytometry analysis.
Get your neural cells from adult rodent brain in only four hours.

  • Gentle and efficient dissociation of adult rodent brain
  • Fast and easy isolation of primary adult neural cells
  • Consistent and superior cell culture of neural cells

Gentle brain dissociation

Pure, viable, and functional adult neural cells provide an important tool for fully understanding the biology of adult neural cells, disease mechanisms, and for drug screening. The gentleMACS™ Octo Dissociator with Heaters and the MACS® Adult Brain Dissociation Kit are optimized for fully automated and standardized dissociation of adult rodent brains older than P7. Adult rodent brains are dissociated gently yet efficiently to obtain maximum yields of viable cells, including neurons, astrocytes, microglia, oligodendrocytes, and endothelial cells. The dissociated cells are immediately ready for cell separation or any other downstream application.

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Isolation of adult neural cells

By using MACS® Technology, various types of adult neural cells can be isolated directly after adult brain dissociation:
  • Astrocytes: using the Anti-ACSA-2 MicroBead Kit, mouse adult astrocytes can be isolated with >98% purity
  • Oligodendrocytes: using Anti-O4 MicroBeads, mouse, the pre-mature oligodendrocytes can be isolated with >90% purity
  • Neurons: using the Neuron Isolation Kit, mouse the neuronal cells can be isolated with >90% purity
  • Microglia: using CD11b (Microglia) MicroBeads, human and mouse or CD11b/c (microglia), rat, microglia can be isolated with >96% purity.
  • Endothelial cells: using CD45 MicroBeads and CD31 MicroBeads, endothelial cells can be isolated with >96% purity

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Cultivation of adult neural cells

Cultivation of neural cells can be a challenging task. The MACS® Neuro-brew 21, together with the MACS Neuro Medium, are specifically optimized for neural cell culture.

Educational material

Flow cytometry tools for analysis of neural cells

With our huge variety of MACS® Antibodies, including the recombinantly engineered REAfinity™ Antibodies, adult neural cells as well as brain infiltrated cells can be easily analyzed by flow cytometry.


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autoMACS Pro Separator - Starter Kit

Components:
  • autoMACS Pro Separator Instrument
  • MACS MiniSampler
  • MACS Reagent Rack 4 (130-094-574)
  • Chill 5, 15, 50 Rack Set (130-097-038)
  • barcode reader
  • software
  • autoMACS® Columns (130-021-101)
  • 4×column substitute
  • autoMACS Pro Buffer Combination
  • User manual
  • short instructions
  • one-year warranty
 
130-092-545 price on request here

gentleMACS Octo Dissociator with Heaters

Components:
  • gentleMACS Octo Dissociator with Heaters
  • Power cord
  • User manual
 
130-096-427 price on request here

MACSQuant Analyzer 10

Content: 1 unit
 
130-096-343 price on request here

Adult Brain Dissociation Kit, mouse and rat

Capacity: for 50 preparations
 
130-107-677 $1,065.00

Anti-ACSA-2 MicroBead Kit, mouse

Capacity: for 1×109 total cells
 
130-097-678 $585.00

Anti-ACSA-2 MicroBead Kit, mouse – small size

Capacity: for 5×107 total cells
 
130-097-679 $75.00

Anti-ACSA-2-PE, mouse (clone: IH3-18A3)

For research use only
Content: 30 µg in 1 mL (1)

Quantity

(1)200 tests. One test corresponds to labeling of up to 106 cells in a final volume of 50 µL.
 
130-102-365 $55.00

Anti-ACSA-2-APC, mouse (clone: IH3-18A3)

For research use only
Content: 30 µg in 1 mL (1)

Quantity

(1)200 tests. One test corresponds to labeling of up to 106 cells in a final volume of 50 µL.
 
130-102-315 $80.00

Anti-ACSA-2-APC, mouse (clone: IH3-18A3)

For research use only
Content: 30 µg in 200 µL (1)

Quantity

(1)100 tests. One test corresponds to labeling of up to 106 cells in a final volume of 100 µL.
 
130-117-535 price on request here

Anti-ACSA-2-APC, mouse (clone: IH3-18A3)

For research use only
Content: 150 µg in 1 mL (2)

Quantity

(2)500 tests. One test corresponds to labeling of up to 106 cells in a final volume of 100 µL.
 
130-117-386 price on request here
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Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 877 591 1060
macs@miltenyibiotec.com
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