Research areas - Application Spotlight - Regulatory T cells

How to work with regulatory T cells

Regardless which aim you have in mind for your regulatory T (Treg) cell approach, we offer workflow solutions from basic to clinical research

  • Harmonized Treg cell product portfolio to fit your research needs
  • Optimized tools for Treg cell separation, expansion and suppression assays
  • Culture isolated Treg cells with standardized medium and cytokines up to GMP grade

Gentle tissue dissociation

The success of an experiment depends on the quality of the starting material and its preparation. Choose the gentleMACS™ Octo Dissociator with Heaters for fully automated and standardized tissue dissociation for up to eight samples in parallel.  The gentleMACS Dissociators and the MACS® Tissue Dissociation Kits are optimized to work together to obtain single-cell suspension with a high viability rate and preserved surface epitopes, which is important for downstream applications.

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Isolation of Treg cells

No matter if you want to isolate regulatory T (Treg) cells from human, mouse or non-human primate sources Miltenyi Biotec is offering optimized products for those species. Choose from our broad Treg cell portfolio, which offers you a variety of possibilities: isolation of Treg cells directly from whole blood, pre-enrichment of CD4+ cells (positive and negative selection), ready-to-use Treg isolation kits, and clinical scaled CD25+ cell enrichment. As always, the proven and gentle technology of MACS® MicroBeads as well as the MACSxpress® Technology ensure unaltered, viable enriched cells with outstanding purity and recovery, immediately ready for any downstream application.

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Treg cell culture tools

Activation, expansion and stimulation of regulatory T (Treg) cells can be challenging. However, now you can trust our cell culture tools that are specially optimized for Treg cell culture. TexMACS™ Medium, MACS® Cytokines and Treg cell expansion tools are all available in research and GMP grade, for seamless translation into (pre-)clinical research.

Educational material

Treg flow analysis

The analysis tools of Miltenyi Biotec’s regulatory T (Treg) cell portfolio consist of a huge variety of MACS® Antibodies and REAfinity™ antibodies for Treg cell specific cell surface markers as well as optimized ready-to-use Treg detection kits (mouse and human). It also offers an ideal solution for the functional analysis of human Treg cells: the Treg Suppression Inspector. When optimizing an experiment from beginning to end complementary flow analysis tools with advanced cell isolation and cell culture products lead you one step closer to reliable and reproducible results.

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gentleMACS Octo Dissociator with Heaters

Components:
  • gentleMACS Octo Dissociator with Heaters
  • Power cord
  • User manual
 
130-096-427 price on request here

Anti-CCR10-PE, human (clone: REA326)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
130-104-868 $95.00

Anti-CCR10-PE, human (clone: REA326)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
130-104-822 $270.00

Anti-CCR10-APC, human (clone: REA326)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
130-104-867 $120.00

Anti-CCR10-APC, human (clone: REA326)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 107 cells in a total volume of 100 µL.
 
130-104-821 $280.00

Anti-FoxP3-PE, human and mouse (clone: 3G3)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 106 cells in a total volume of 100 µL.
 
130-098-119 $100.00

Anti-FoxP3-PE, human and mouse (clone: 3G3)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 106 cells in a total volume of 100 µL.
 
130-093-014 $270.00

Anti-FoxP3-APC, human and mouse (clone: 3G3)

For research use only
Capacity: for 30 tests (1)

Capacity

(1)One test corresponds to labeling of up to 106 cells in a total volume of 100 µL.
 
130-098-121 $120.00

Anti-FoxP3-APC, human and mouse (clone: 3G3)

For research use only
Capacity: for 100 tests (1)

Capacity

(1)One test corresponds to labeling of up to 106 cells in a total volume of 100 µL.
 
130-093-013 $280.00

Anti-FR4-PE, mouse (clone: TH6)

For research use only
Content: 30 µg in 1 mL (1)

Quantity

(1)200 tests. One test corresponds to labeling of up to 106 cells in a final volume of 50 µL.
 
130-102-455 $50.00
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Phone: +1 800 FOR MACS
Fax:+1 877 591 1060
macs@miltenyibiotec.com
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