Products and Services - MACSmolecular - Transfection and transduction - MACSfectin™ Reagent

MACSfectin™ Reagent

  • Efficient: higher transfection efficiency than standard lipid transfection reagents
  • Versatile: transfection of plasmid DNA, mRNA, siRNA
  • Reliable: successfully tested in over 30 cell types, including mouse ES cells

Overview

MACSfectin™ Reagent enables optimized transfection of plasmid DNA for a wide range of adherent and suspension cells in immunology, cancer, stem cell, and neuroscience research. Additionally, MACSfectin Reagent supports tranfection with RNA such as mRNA or siRNA. This unique transfection reagent is based on a novel class of cationic lipopolyamines designed to optimize nucleic acid condensation and augment the cytoplasmic release of the nucleic acid cargo. Additionally, good transgene expression with high cell viability is achieved due to MACSfectin Reagent’s excellent biodegradation characteristics.

Details

Applications

MACSfectin Reagent can be applied for mammalian cell transfection using plasmid DNA, mRNA, and siRNA. Possible downstream applications after transfection with MACSfectin Reagent are protein expression and purification, protein interaction studies, reporter gene assays, fluorescent imaging, gene knock-down, and generation of stable cell lines.

Gallery

Figure 1

Figure 1
Overview of MACSfectin Reagent transfection protocol.
Overview of MACSfectin Reagent transfection protocol.

Figure 2

Figure 2
Efficient transfection using MACSfectin™ Reagent. 10 adherent cell lines (A) and 6 suspension cell lines (B) were transfected with a plasmid vector expressing cell surface human CD4 in 6-well plates with MACSfectin Reagent and a competitor product using standard transfection conditions (4 µg DNA and 8–16 µL MACSfectin Reagent). The number of transfected cells and mean fluorescence intensity (MFI) were assessed 48 hours post-transfection by staining the cells with CD4-APC and flow cytometric analysis using a MACSQuant® Analyzer. The MFI value of cells transfected with MACSfectin Reagent were set to 100% for comparison with the competitor product.
Efficient transfection using MACSfectin™ Reagent. 10 adherent cell lines (A) and 6 suspension cell lines (B) were transfected with a plasmid vector expressing cell surface human CD4 in 6-well plates with MACSfectin Reagent and a competitor product using standard transfection conditions (4 µg DNA and 8–16 µL MACSfectin Reagent). The number of transfected cells and mean fluorescence intensity (MFI) were assessed 48 hours post-transfection by staining the cells with CD4-APC and flow cytometric analysis using a MACSQuant® Analyzer. The MFI value of cells transfected with MACSfectin Reagent were set to 100% for comparison with the competitor product.

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Product Order no. Price

MACSfectin™ Reagent

Capacity: for up to 500 transfections (1)
Content: 0.5 mL

Capacity

(1)in 24-well plates.
 
Data sheet
130-098-410 $255.00

MACSfectin™ Reagent

Capacity: for up to 1000 transfections (1)
Content: 1 mL

Capacity

(1)in 24-well plates.
 
Data sheet
130-098-411 $420.00

MACSfectin™ Reagent

Capacity: for up to 5000 transfections (1)
Content: 5×1 mL

Capacity

(1)in 24-well plates.
 
Data sheet
130-098-412 $1,665.00

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Miltenyi Biotec Inc.
Phone: +1 800 FOR MACS
Fax:+1 877 591 1060
macs@miltenyibiotec.com
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