Genetic manipulation of cells by transfection has become a standard technique in many life science laboratories. MACSfectin™ Reagent is applicable for plasmid DNA, mRNA, and siRNA transfection. The reagent is based on a novel class of cationic lipopolyamines that optimize nucleic acid condensation while augmenting the cytoplasmic release of the nucleic acid cargo. MACSfectin Reagent exhibits high biodegradation characteristics resulting in good transgene expression with high cell viability. MACSfectin can be used for transfection of a wide range of adherent and suspension cells utilized in immunology, cancer, stem cell, and neuroscience research.
The MACSelect™ System, which is based on the renowned MACS® Technology, helps to tackle low transfection efficiency via magnetic enrichment of transfected cells. Cumbersome stable transfection and antibiotic selection is no longer needed. The system uses the transiently expressed truncated human CD4 molecule, the truncated mouse MHC class I molecule H-2Kk, and the truncated human low-affinity nerve growth factor receptor (LNGFR) as surface markers to select transfected cells. It is applicable to all adherent or suspension cell lines and primary cells lacking the selection markers in combination with virtually any transfection method. The MACSelect System has been widely applied in immunology, cancer, stem cell, and neuroscience research for cell enrichment after transient transfection and help to significantly increase the sensitivity of downstream analyses.
A higher expression efficiency of introduced genes can also be achieved using viral vectors. This approach is highly beneficial when dealing with difficult-to-transfect primary cells and cell lines. Standard transduction methods, however, result in relatively low transduction efficiencies as they rely on passive diffusion of virus particles to the cell surface. MACSductin™ Reagent consists of polycationic, magnetic beads that help to efficiently transduce primary cells and cell lines using adeno- or retro-/lentiviral vectors. In combination with MACS Technology MACSductin Reagent enables close co-localization of virus and target cells within MACS Columns. This leads to an increase in transduction efficiency compared to standard viral transduction methods.
Download Cell Transfection and Viral Transduction Flyer