Mitochondria research quite often requires well-preserved mitochondria preparations for functional analysis. In addition to primary cells or cell lines human or mouse tissue samples are the major mitochondria sources. For the effective isolation of functional mitochondria, appropriate procedures for tissue homogenization and subsequent cell lysis are crucial. The Mitochondria Extraction Kit – Tissue, in combination with the gentleMACS™ Dissociators, offers standardized one-step mitochondria extraction, including tissue homogenization and cell lysis. This procedure results in the optimal tissue homogenate for the subsequent isolation of functional and pure mitochondria with the Mitochondria Isolation Kit, human or Mitochondria Isolation Kit, mouse tissue.
Exosomes are secreted by a variety of cell types of hematopoietic and non-hematopoietic origin into diverse body fluids. The surface protein composition of extracellular vesicles (EVs) is related to the originating cell and may play a role in vesicle function. The Exosome Isolation Kits, human and mouse facilitate the specific isolation of intact exosomes or EVs from cell culture supernatant, plasma, urine, or ascites. Isolated EVs can be immediately analyzed by flow cytometry, for example, with the MACSPlex Exosome Kit, human.
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